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Anti-GRP75/HSPA9 Antibody (Clone#OTI9F8)

Mouse monoclonal antibody

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M02561-1

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 5-7天
  • Flow cytometric Analysis of Hela cells, using anti-HSPA9 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).

    all(13)
  • Flow cytometric Analysis of Jurkat cells, using anti-HSPA9 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).

    all(13)
  • Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-HSPA9 monoclonal antibody (1:200).

    all(13)
  • Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-HSPA9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02561-1)

    all(13)
  • Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-HSPA9 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

    all(13)
  • Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-HSPA9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02561-1)

    all(13)
  • Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-HSPA9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02561-1)

    all(13)
  • Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-HSPA9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02561-1)

    all(13)
  • Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-HSPA9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02561-1)

    all(13)
  • Immunofluorescent staining of HepG2 cells using anti-HSPA9 mouse monoclonal antibody.

    all(13)
  • Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-HSPA9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02561-1)

    all(13)
  • Anti-HSPA9 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HSPA9 .

    all(13)
  • HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPA9 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSPA9(Cat# M02561-1).

    all(13)

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产品简介>

产品名称
Anti-GRP75/HSPA9 Antibody (Clone#OTI9F8)
规格/价格
50μl|1280 100μl|2180 150μl|2800
指标别称
CSA; GRP 75; GRP75; Heat shock 70 kDa protein 9; HSPA9; HSPA9B; Mortalin; MOT; mot 2; MOT2; MTHSP75; PBP74; Peptide binding protein 74
产品类型
Monoclonal
检验物种
human, mouse, rat, dog, monkey
应用范围
WB, IHC, ICC/IF, FCM
基因名称
HSPA9
克隆号
OTI9F8
宿主
Mouse
抗体亚型
IgG2b
免疫原
Full length human recombinant protein of human HSPA9 (NP_004125) produced in HEK293T cell.
计算分子量
74 kDa
实际分子量
74 kDa
成分
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
纯化方式
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
浓度
500 ug/ml
产品形态
Liquid
保存条件
Stable for 12 months from date of receipt. Store at -20°C as received.
背景资料
This gene encodes a member of the heat shock protein 70 gene family. The encoded protein is primarily localized to the mitochondria but is also found in the endoplasmic reticulum, plasma membrane and cytoplasmic vesicles. This protein is a heat-shock cognate protein. This protein plays a role in cell proliferation, stress response and maintenance of the mitochondria. A pseudogene of this gene is found on chromosome 2.
Uniprot ID
P38646  
文献引用格式
GRP75/HSPA9 Antibody (Clone#OTI9F8) (Boster Biological Technology, Wuhan, China. Catalog#M02561-1)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
基因名全称
heat shock 70kDa protein 9 (mortalin)
蛋白名全称
Stress-70 protein, mitochondrial
推荐稀释比
Western blot (WB):1:1000~2000
Immunohistochemistry (IHC):1:50
Immunocytochemistry/Immunofluorescence (ICC/IF):1:50
Flow cytometry (FCM):1:100

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