Anti-NONO Antibody (Clone#11E2)

筛选器： All WB FCM ICC/IF

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  Western blot analysis of NONO using anti-NONO antibody (M03515). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human HELA whole cell lysates,
  Lane 2: human Placent tissue lysates,
  Lane 3: human MCF-7 whole cell lysates,
  Lane 4: human A549 whole cell lysates,
  Lane 5: human SW620 whole cell lysates,
  Lane 6: human PANC-1 whole cell lysates,
  Lane 7: human U2OS whole cell lysates,
  Lane 8: human K562 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-NONO antigen affinity purified monoclonal antibody (M03515) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NONO at approximately 60 kDa. The expected band size for NONO is at 54 kDa.

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  Flow Cytometry analysis of U2OS cells using anti-nmt55 p54nrb antibody (M03515).Overlay histogram showing U2OS cells stained with M03515 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-nmt55 p54nrb Antibody (M03515, 1:100) for 30 min at 20°C. Fluoro®488 conjugated goat anti-mouse IgG (BA1126, 1:100) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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  ICC analysis of NONO using anti- NONO antibody (M03515).
  NONO was detected in an immunocytochemical section of A431 cells. The section was incubated with mouse anti-NONO Antibody (M03515) at a dilution of 1:100. Biotinylated goat anti-mouse IgG was used as secondary antibody. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.

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  ICC/IF analysis of NONO using anti-NONO antibody (M03515).
  NONO was detected in an immunocytochemical section of MCF-7 cells. The section was incubated with mouse anti-NONO Antibody (M03515) at a dilution of 1:100. Fluoro488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog#BA1126) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

• 

  Western blot analysis of NONO using anti-NONO antibody (M03515). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human HELA whole cell lysates,
  Lane 2: human Placent tissue lysates,
  Lane 3: human MCF-7 whole cell lysates,
  Lane 4: human A549 whole cell lysates,
  Lane 5: human SW620 whole cell lysates,
  Lane 6: human PANC-1 whole cell lysates,
  Lane 7: human U2OS whole cell lysates,
  Lane 8: human K562 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-NONO antigen affinity purified monoclonal antibody (M03515) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NONO at approximately 60 kDa. The expected band size for NONO is at 54 kDa.

• 

  Flow Cytometry analysis of U2OS cells using anti-nmt55 p54nrb antibody (M03515).Overlay histogram showing U2OS cells stained with M03515 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-nmt55 p54nrb Antibody (M03515, 1:100) for 30 min at 20°C. Fluoro®488 conjugated goat anti-mouse IgG (BA1126, 1:100) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

• 

  ICC analysis of NONO using anti- NONO antibody (M03515).
  NONO was detected in an immunocytochemical section of A431 cells. The section was incubated with mouse anti-NONO Antibody (M03515) at a dilution of 1:100. Biotinylated goat anti-mouse IgG was used as secondary antibody. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.

• 

  ICC/IF analysis of NONO using anti-NONO antibody (M03515).
  NONO was detected in an immunocytochemical section of MCF-7 cells. The section was incubated with mouse anti-NONO Antibody (M03515) at a dilution of 1:100. Fluoro488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog#BA1126) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).