| Western blot (WB): | 1:2000 |
| Immunohistochemistry (IHC): | 1:150 |
| Flow cytometry (FCM): | 1:100 |

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MME (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MME (Cat# M04065-3)(1:500).

Western blot analysis of extracts (35ug) from 1 tissue lysate by using anti-MME monoclonal antibody (1:500).

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-MME mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M04065-3)

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-MME mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M04065-3)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-MME mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M04065-3)

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-MME mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M04065-3)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MME mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M04065-3)

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MME mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M04065-3)

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-MME mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M04065-3)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MME mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M04065-3)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-MME mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M04065-3)

Flow cytometric analysis of living 293T cells transfected with MME overexpression plasmid , Red)/empty vector (Blue) using anti-MME antibody. Cells incubated with a non-specific antibody (Green) were used as isotype control (1:100).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MME (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MME (Cat# M04065-3)(1:500).

Western blot analysis of extracts (35ug) from 1 tissue lysate by using anti-MME monoclonal antibody (1:500).

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-MME mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M04065-3)

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-MME mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M04065-3)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-MME mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M04065-3)

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-MME mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M04065-3)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MME mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M04065-3)

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MME mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M04065-3)

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-MME mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M04065-3)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MME mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M04065-3)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-MME mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M04065-3)

Flow cytometric analysis of living 293T cells transfected with MME overexpression plasmid , Red)/empty vector (Blue) using anti-MME antibody. Cells incubated with a non-specific antibody (Green) were used as isotype control (1:100).











