Anti-CD23/FCER2 Antibody (Clone#OTI2B6)

筛选器： All WB IHC FCM

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FCER2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FCER2 (Cat# MA04237)(1:2000).

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  Western blot analysis of extracts (35ug) from 2 cell lines lysates by using anti-FCER2 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-FCER2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, MA04237) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-FCER2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, MA04237) (1:150)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-FCER2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, MA04237) (1:150)

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  Flow cytometric analysis of living 293T cells transfected with FCER2 overexpression plasmid , Red)/empty vector (Blue) using anti-FCER2 antibody. Cells incubated with a non-specific antibody (Green) were used as isotype control (1:100).

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  Flow cytometric analysis of living RPMI-8226 cells, using anti-FCER2 antibody, compared to an isotype control (green), and a PBS control (blue) (1:100).

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FCER2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FCER2 (Cat# MA04237)(1:2000).

• 

  Western blot analysis of extracts (35ug) from 2 cell lines lysates by using anti-FCER2 monoclonal antibody (1:500).

• 

  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-FCER2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, MA04237) (1:500)

• 

  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-FCER2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, MA04237) (1:150)

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-FCER2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, MA04237) (1:150)

• 

  Flow cytometric analysis of living 293T cells transfected with FCER2 overexpression plasmid , Red)/empty vector (Blue) using anti-FCER2 antibody. Cells incubated with a non-specific antibody (Green) were used as isotype control (1:100).

• 

  Flow cytometric analysis of living RPMI-8226 cells, using anti-FCER2 antibody, compared to an isotype control (green), and a PBS control (blue) (1:100).