Anti-ACAA2 Antibody (Clone#26A90)

Rabbit monoclonal antibody

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筛选器： All WB IHC ICC/IF

M08341-1

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-ACAA2 antibody (M08341-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human PANC-1 whole cell lysates,
Lane 3: human Caco-2 whole cell lysates,
Lane 4: human U2OS whole cell lysates,
Lane 5: rat heart tissue lysates,
Lane 6: rat kidney tissue lysates,
Lane 7: mouse heart tissue lysates,
Lane 8: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ACAA2 antigen affinity purified monoclonal antibody (M08341-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ACAA2 at approximately 42 kDa. The expected band size for ACAA2 is at 42 kDa.
all(6)
IHC analysis of ACAA2 using anti-ACAA2 antibody (M08341-1) .
ACAA2 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. The tissue section was incubated with rabbit anti-ACAA2 Antibody (M08341-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(6)
IHC analysis of ACAA2 using anti-ACAA2 antibody (M08341-1) .
ACAA2 was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was incubated with rabbit anti-ACAA2 Antibody (M08341-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(6)
IHC analysis of ACAA2 using anti-ACAA2 antibody (M08341-1) .
ACAA2 was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was incubated with rabbit anti-ACAA2 Antibody (M08341-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(6)
IHC analysis of ACAA2 using anti-ACAA2 antibody (M08341-1) .
ACAA2 was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-ACAA2 Antibody (M08341-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(6)
IF analysis of ACAA2 using anti-ACAA2 antibody (M08341-1).
ACAA2 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-ACAA2 Antibody (M08341-1) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
all(6)

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产品简介>

产品名称

Anti-ACAA2 Antibody (Clone#26A90)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

ACAA2; Acetyl CoA acyltransferase; Beta ketothiolase; DSAEC; T1

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF

基因名称

ACAA2

克隆号

26A90

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human ACAA2

计算分子量

42 kDa

实际分子量

42 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

3-Ketoacyl-CoA thiolase, mitochondrial, also known as acetyl-Coenzyme A acyltransferase 2, is an acetyl-CoA C-acyltransferase enzyme that in humans is encoded by the ACAA2 gene. The ACAA2 gene encodes a 41.9 kDa protein that is composed of 397 amino acids and contains 88 observed peptides. The encoded protein catalyzes the last step of themitochondrial fatty acid beta oxidation spiral. Unlike most mitochondrial matrix proteins, it contains a non-cleavable amino-terminal targeting signal. Additionally, ACAA2 has been shown to be a functional BNIP3 binding partner, which provides a possible link between fatty acid metabolism and cell apoptosis.

Uniprot ID

P42765

文献引用格式

ACAA2 Antibody (Clone#26A90) (Boster Biological Technology, Wuhan, China. Catalog#M08341-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

acetyl-CoA acyltransferase 2

蛋白名全称

3-ketoacyl-CoA thiolase, mitochondrial

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实验步骤

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