Western blot (WB): | 1:500-2000 |
Western blot analysis of Fractalkine/CX3CL1 using anti-Fractalkine/CX3CL1 antibody (PA1400).
Lane 1: recombinant human Fractalkine protein 10ng,
Lane 2: recombinant human Fractalkine protein 5ng,
Lane 3: recombinant human Fractalkine protein 2.5ng.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Fractalkine/CX3CL1 antigen affinity purified polyclonal antibody (PA1400) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Fractalkine/CX3CL1 at approximately 90-100 kDa.
Western blot analysis of Fractalkine/CX3CL1 using anti-Fractalkine/CX3CL1 antibody (PA1400).
Lane 1: recombinant human Fractalkine protein 10ng,
Lane 2: recombinant human Fractalkine protein 5ng,
Lane 3: recombinant human Fractalkine protein 2.5ng.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Fractalkine/CX3CL1 antigen affinity purified polyclonal antibody (PA1400) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Fractalkine/CX3CL1 at approximately 90-100 kDa.