Anti-Complement C5 Antibody
送常用试剂

Rabbit polyclonal antibody

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筛选器： All IHC WB

PA2308

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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IHC analysis of Complement C5 using anti-Complement C5 antibody (PA2308).
Complement C5 was detected in a paraffin-embedded section of mouse spleen tissue. The tissue section was incubated with rabbit anti-Complement C5 Antibody (PA2308) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(4)
IHC analysis of Complement C5 using anti-Complement C5 antibody (PA2308).
Complement C5 was detected in a paraffin-embedded section of rat liver tissue. The tissue section was incubated with rabbit anti-Complement C5 Antibody (PA2308) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(4)
IHC analysis of Complement C5 using anti-Complement C5 antibody (PA2308).
Complement C5 was detected in a paraffin-embedded section of rat spleen tissue. The tissue section was incubated with rabbit anti-Complement C5 Antibody (PA2308) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(4)
Western blot analysis of Complement C5 using anti-Complement C5 antibody (PA2308). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat Liver tissue lysates at 40ug,
Lane 2: Mouse Liver tissue lysates at40ug,
Lane 3: NIH whole cell lysates at 40ug,
Lane 4: HEPA whole cell lysates at 40ug,
Lane 5: PC12 whole cell lysates at 40ug.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Complement C5 antigen affinity purified polyclonal antibody (PA2308) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Complement C5 at approximately 75 kDa/115 kDa. The expected band size for Complement C5 is at 9 kDa.
all(4)

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产品简介>

产品名称

Anti-Complement C5 Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

C5; Complement C5; complement component 5; CPAMD4

产品类型

Polyclonal

检验物种

mouse, rat

应用范围

IHC, WB

基因名称

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the N-terminus of rat C5a, different from the related mouse sequence by four amino acids.

计算分子量

9 kDa

实际分子量

75 kDa/115 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

C5a is a protein fragment released from complement component C5. This gene is mapped to 9q33.2. The protein encoded by this gene is the fifth component of complement, which plays an important role in inflammatory and cell killing processes. This protein is comprised of alpha and beta polypeptide chains that are linked by a disulfide bridge. An activation peptide, C5a, which is an anaphylatoxin that possesses potent spasmogenic and chemotactic activity, is derived from the alpha polypeptide via cleavage with a convertase. The C5b macromolecular cleavage product can form a complex with the C6 complement component, and this complex is the basis for formation of the membrane attack complex, which includes additional complement components. Mutations in this gene cause complement component 5 deficiency, a disease where patients show a propensity for severe recurrent infections. Defects in this gene have also been linked to susceptibility to liver fibrosis and to rheumatoid arthritis.

Uniprot ID

P08650

RRID

AB_3082925

文献引用格式

Complement C5 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PA2308)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

complement component 5

蛋白名全称

Complement C5

实验方案>

实验步骤

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