Western blot (WB): | 1:500-2000 |
Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |
Western blot analysis of IL15 using anti-IL15 antibody (PB0249).
Lane 1: recombinant Human IL15 Protein 0.5ng.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-IL15 antigen affinity purified polyclonal antibody (PB0249) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for IL15 at approximately 15 kDa.
Western blot analysis of IL15 using anti-IL15 antibody (PB0249).
Lane 1: recombinant Human IL15 Protein 0.5ng.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-IL15 antigen affinity purified polyclonal antibody (PB0249) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for IL15 at approximately 15 kDa.