Immunohistochemistry (IHC): | 1:50-400 |
Immunofluorescence (IF): | 1:50-400 |
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
IHC analysis of Glucagon/GCG using anti-Glucagon/GCG antibody (PB0742).
Glucagon/GCG was detected in a paraffin-embedded section of mouse pancreas tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Glucagon/GCG Antibody (PB0742) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Glucagon/GCG using anti-Glucagon/GCG antibody (PB0742).
Glucagon/GCG was detected in a paraffin-embedded section of rat pancreas tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Glucagon/GCG Antibody (PB0742) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Glucagon/GCG using anti-Glucagon/GCG antibody (PB0742).
Glucagon/GCG was detected in a paraffin-embedded section of human pancreatic cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Glucagon/GCG Antibody (PB0742) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of Glucagon/GCG using anti-Glucagon/GCG antibody (PB0742) and anti-Insulin/INS antibody (BM0080).
Glucagon/GCG was detected in a paraffin-embedded section of mouse pancreas tissue. The tissue section was incubated with rabbit anti-Glucagon/GCG Antibody (PB0742) at a dilution of 1:100. Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red)(Catalog#BA1031) were used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
IF analysis of Glucagon/GCG using anti-Glucagon/GCG antibody (PB0742) and anti-Insulin/INS antibody (BM0080).
Glucagon/GCG was detected in a paraffin-embedded section of rat pancreas tissue. The tissue section was incubated with rabbit anti-Glucagon/GCG Antibody (PB0742) at a dilution of 1:100. Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red)(Catalog#BA1031) were used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
IHC analysis of Glucagon/GCG using anti-Glucagon/GCG antibody (PB0742).
Glucagon/GCG was detected in a paraffin-embedded section of mouse pancreas tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Glucagon/GCG Antibody (PB0742) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Glucagon/GCG using anti-Glucagon/GCG antibody (PB0742).
Glucagon/GCG was detected in a paraffin-embedded section of rat pancreas tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Glucagon/GCG Antibody (PB0742) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Glucagon/GCG using anti-Glucagon/GCG antibody (PB0742).
Glucagon/GCG was detected in a paraffin-embedded section of human pancreatic cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Glucagon/GCG Antibody (PB0742) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of Glucagon/GCG using anti-Glucagon/GCG antibody (PB0742) and anti-Insulin/INS antibody (BM0080).
Glucagon/GCG was detected in a paraffin-embedded section of mouse pancreas tissue. The tissue section was incubated with rabbit anti-Glucagon/GCG Antibody (PB0742) at a dilution of 1:100. Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red)(Catalog#BA1031) were used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
IF analysis of Glucagon/GCG using anti-Glucagon/GCG antibody (PB0742) and anti-Insulin/INS antibody (BM0080).
Glucagon/GCG was detected in a paraffin-embedded section of rat pancreas tissue. The tissue section was incubated with rabbit anti-Glucagon/GCG Antibody (PB0742) at a dilution of 1:100. Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red)(Catalog#BA1031) were used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).