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Anti-Glucagon/GCG Antibody

Rabbit polyclonal antibody

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筛选器: All IHC IF

PB0742

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • IHC analysis of Glucagon/GCG using anti-Glucagon/GCG antibody (PB0742).
    Glucagon/GCG was detected in a paraffin-embedded section of mouse pancreas tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Glucagon/GCG Antibody (PB0742) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

    all(5)
  • IHC analysis of Glucagon/GCG using anti-Glucagon/GCG antibody (PB0742).
    Glucagon/GCG was detected in a paraffin-embedded section of rat pancreas tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Glucagon/GCG Antibody (PB0742) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

    all(5)
  • IHC analysis of Glucagon/GCG using anti-Glucagon/GCG antibody (PB0742).
    Glucagon/GCG was detected in a paraffin-embedded section of human pancreatic cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Glucagon/GCG Antibody (PB0742) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

    all(5)
  • IF analysis of Glucagon/GCG using anti-Glucagon/GCG antibody (PB0742) and anti-Insulin/INS antibody (BM0080).
    Glucagon/GCG was detected in a paraffin-embedded section of mouse pancreas tissue. The tissue section was incubated with rabbit anti-Glucagon/GCG Antibody (PB0742) at a dilution of 1:100. Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red)(Catalog#BA1031) were used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

    all(5)
  • IF analysis of Glucagon/GCG using anti-Glucagon/GCG antibody (PB0742) and anti-Insulin/INS antibody (BM0080).
    Glucagon/GCG was detected in a paraffin-embedded section of rat pancreas tissue. The tissue section was incubated with rabbit anti-Glucagon/GCG Antibody (PB0742) at a dilution of 1:100. Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red)(Catalog#BA1031) were used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

    all(5)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称
Anti-Glucagon/GCG Antibody
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
GCG; GLP 2; GLP1; GLP2; glucagon; GRPP; GRPP Oxyntomodulin; OXM; Proglucagon
产品类型
Polyclonal
检验物种
human, mouse, rat
应用范围
IHC, IF
基因名称
GCG
宿主
Rabbit
抗体亚型
IgG
免疫原
A synthetic peptide corresponding to a sequence at the N-terminus of human GLP1 identical to the related mouse and rat sequences.
成分
500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied.
背景资料
GCG is also known as GLP1, or Glucagon. Glucagon is a 29-amino acid pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulating glycogenolysis and gluconeogenesis. It is mapped to 2q36-2q37. GLP1, also known as 7-37 for the codons of the preproglucagon molecule which encode it, renders pancreatic beta-cells 'glucose-competent' and may be useful in the treatment of noninsulin-dependent diabetes mellitus. Also, GLP1 is a potent insulin secretagogue. It plays a major role in the enteroinsular axis, accounting, for example, for the finding that plasma insulin levels accompanying oral intake of glucose are greater than those observed when glucose is given intravenously.
Uniprot ID
P01275  
文献引用格式
Glucagon/GCG Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB0742)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。
基因名全称
glucagon
蛋白名全称
Glucagon
推荐稀释比
Immunohistochemistry (IHC):1:50-400
Immunofluorescence (IF):1:50-400
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

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    Anti-Glucagon/GCG Antibody

    筛选器: All IHC IF

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