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Anti-IP10/CXCL10 Antibody

Rabbit polyclonal antibody

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筛选器: All WB IHC ELISA

PB0752

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Western blot analysis of IP10/CXCL10 using anti-IP10/CXCL10 antibody (PB0752).
    Lane 1: recombinant mouse CXCL10 protein 10ng.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-IP10/CXCL10 antigen affinity purified polyclonal antibody (PB0752) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for IP10/CXCL10 at approximately 11 kDa.

    all(3)
  • IHC analysis of IP10/CXCL10 using anti-IP10/CXCL10 antibody (PB0752) .
    IP10/CXCL10 was detected in a paraffin-embedded section of mouse spleen tissue. The tissue section was incubated with rabbit anti-IP10/CXCL10 Antibody (PB0752) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(3)

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产品名称
Anti-IP10/CXCL10 Antibody
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
C X C motif chemokine 10; C7; crg 2; CXCL10; CXCL10/IP10; Gamma IP10; gIP 10; IFI10; INP10; IP 10; IP10; mob 1; SCYB10; Small inducible cytokine B10
产品类型
Polyclonal
检验物种
mouse
应用范围
WB, IHC, ELISA
基因名称
CXCL10
宿主
Rabbit
抗体亚型
IgG
免疫原
E. coli-derived mouse IP10 recombinant protein (Position: I22-P98). Mouse IP10 shares 70.1% and 77.9% amino acid (aa) sequence identity with human and rat IP10, respectively.
计算分子量
11 kDa
实际分子量
11 kDa
成分
500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied.
背景资料
C-X-C motif chemokine 10 (CXCL10), also known as Interferon gamma-induced protein 10 kDa (IP-10) or small-inducible cytokine B10, is a protein that in humans is encoded by the CXCL10 gene. It is a small cytokine belonging to the CXC chemokine family. The gene for CXCL10 is located on human chromosome 4 in a cluster among several other CXC chemokines. Luster et al. (1987) reported the isolation of an interferon-inducible gene that encodes a 98-amino acid protein called IP10. The chemokine IFN-gamma-inducible protein 10 (IP-10; CXCL10), a CXC chemokine, and its receptor, CXCR3, were found to be overexpressed in lymph node cells of EAMG rats. IP10 inhibits bone marrow colony formation, has antitumor activity in vivo, is a chemoattractant for human monocytes and T cells, and promotes T cell adhesion to endothelial cells.
Uniprot ID
P17515  
文献引用格式
IP10/CXCL10 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB0752)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。
基因名全称
chemokine (C-X-C motif) ligand 10
蛋白名全称
C-X-C motif chemokine 10
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-400
Enzyme linked immunosorbent assay (ELISA):1:100-1000
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

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    Anti-IP10/CXCL10 Antibody

    筛选器: All WB IHC ELISA

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