Western blot (WB): | 1:500-2000 |
Immunohistochemistry (IHC): | 1:50-400 |
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
Western blot analysis of IP10/CXCL10 using anti-IP10/CXCL10 antibody (BA4723).
Lane 1: recombinant human CXCL10 protein 10ng,
Lane 2: recombinant human CXCL10 protein 5ng,
Lane 3: recombinant human CXCL10 protein 2.5ng.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-IP10/CXCL10 antigen affinity purified polyclonal antibody (BA4723) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for IP10/CXCL10 at approximately 11 kDa.
IHC analysis of IP10/CXCL10 using anti-IP10/CXCL10 antibody (BA4723).
IP10/CXCL10 was detected in a paraffin-embedded section of human mammary cancer tissue. The tissue section was incubated with rabbit anti-IP10/CXCL10 Antibody (BA4723) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
Western blot analysis of IP10/CXCL10 using anti-IP10/CXCL10 antibody (BA4723).
Lane 1: recombinant human CXCL10 protein 10ng,
Lane 2: recombinant human CXCL10 protein 5ng,
Lane 3: recombinant human CXCL10 protein 2.5ng.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-IP10/CXCL10 antigen affinity purified polyclonal antibody (BA4723) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for IP10/CXCL10 at approximately 11 kDa.
IHC analysis of IP10/CXCL10 using anti-IP10/CXCL10 antibody (BA4723).
IP10/CXCL10 was detected in a paraffin-embedded section of human mammary cancer tissue. The tissue section was incubated with rabbit anti-IP10/CXCL10 Antibody (BA4723) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.