Anti-RAB11A Antibody

筛选器： All WB ICC/IF FCM

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  Western blot analysis of RAB11A using anti-RAB11A antibody (PB0836). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human 293T whole cell lysates,
  Lane 2: human SH-SY5Y whole cell lysates,
  Lane 3: human Hela whole cell lysates,
  Lane 4: human MCF-7 whole cell lysates,
  Lane 5: human A549 whole cell lysates,
  Lane 6: human RT4 whole cell lysates,
  Lane 7: human Hacat whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-RAB11A antigA03957-Aen affinity purified polyclonal antibody (PB0836) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for RAB11A at approximately 24 kDa. The expected band size for RAB11A is at 24 kDa.

• 

  Western blot analysis of RAB11A using anti-RAB11A antibody (PB0836). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: rat brain tissue lysates,
  Lane 2: rat spleen tissue lysates,
  Lane 3: rat lung tissue lysates,
  Lane 4: rat PC-12 whole cell lysates,
  Lane 5: mouse brain tissue lysates,
  Lane 6: mouse spleen tissue lysates,
  Lane 7: mouse lung tissue lysates,
  Lane 8: mouse NIH/3T3 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-RAB11A antigA03957-Aen affinity purified polyclonal antibody (PB0836) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for RAB11A at approximately 24 kDa. The expected band size for RAB11A is at 24 kDa.

• 

  IF analysis of RAB11A using anti-RAB11A antibody (PB0836).
  RAB11A was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-RAB11A Antibody (PB0836) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

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  Flow Cytometry analysis of SH-SY5Y cells using anti-RAB11A antibody (PB0836).
  Overlay histogram showing SH-SY5Y cells stained with PB0836 (Blue line). To facilitate intrRAB11Allular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RAB11A Antibody (PB0836) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

• 

  Western blot analysis of RAB11A using anti-RAB11A antibody (PB0836). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human 293T whole cell lysates,
  Lane 2: human SH-SY5Y whole cell lysates,
  Lane 3: human Hela whole cell lysates,
  Lane 4: human MCF-7 whole cell lysates,
  Lane 5: human A549 whole cell lysates,
  Lane 6: human RT4 whole cell lysates,
  Lane 7: human Hacat whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-RAB11A antigA03957-Aen affinity purified polyclonal antibody (PB0836) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for RAB11A at approximately 24 kDa. The expected band size for RAB11A is at 24 kDa.

• 

  Western blot analysis of RAB11A using anti-RAB11A antibody (PB0836). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: rat brain tissue lysates,
  Lane 2: rat spleen tissue lysates,
  Lane 3: rat lung tissue lysates,
  Lane 4: rat PC-12 whole cell lysates,
  Lane 5: mouse brain tissue lysates,
  Lane 6: mouse spleen tissue lysates,
  Lane 7: mouse lung tissue lysates,
  Lane 8: mouse NIH/3T3 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-RAB11A antigA03957-Aen affinity purified polyclonal antibody (PB0836) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for RAB11A at approximately 24 kDa. The expected band size for RAB11A is at 24 kDa.

• 

  IF analysis of RAB11A using anti-RAB11A antibody (PB0836).
  RAB11A was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-RAB11A Antibody (PB0836) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

• 

  Flow Cytometry analysis of SH-SY5Y cells using anti-RAB11A antibody (PB0836).
  Overlay histogram showing SH-SY5Y cells stained with PB0836 (Blue line). To facilitate intrRAB11Allular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RAB11A Antibody (PB0836) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.