Anti-Band 3/AE1/SLC4A1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC IF FCM

PB9437

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti- SLC4A1/BAND3 antibody (PB9437). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human T-47D whole cell lysates.
Use rabbit anti- SLC4A1/BAND3 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for SLC4A1/BAND3 at approximately 102KD. The expected band size for SLC4A1/BAND3 is at 102KD.
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IHC analysis of Band 3/AE1/SLC4A1 using anti-Band 3/AE1/SLC4A1 antibody (PB9437).
Band 3/AE1/SLC4A1 was detected in a paraffin-embedded section of rat kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Band 3/AE1/SLC4A1 Antibody (PB9437) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(9)
IHC analysis of Band 3/AE1/SLC4A1 using anti-Band 3/AE1/SLC4A1 antibody (PB9437).
Band 3/AE1/SLC4A1 was detected in a paraffin-embedded section of human placenta tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Band 3/AE1/SLC4A1 Antibody (PB9437) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(9)
IHC analysis of Band 3/AE1/SLC4A1 using anti-Band 3/AE1/SLC4A1 antibody (PB9437).
Band 3/AE1/SLC4A1 was detected in a paraffin-embedded section of Mouse Kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Band 3/AE1/SLC4A1 Antibody (PB9437) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(9)
IHC analysis of Band 3/AE1/SLC4A1 using anti-Band 3/AE1/SLC4A1 antibody (PB9437).
Band 3/AE1/SLC4A1 was detected in frozen section of human placenta tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Band 3/AE1/SLC4A1 Antibody (PB9437) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(9)
IHC analysis of Band 3/AE1/SLC4A1 using anti-Band 3/AE1/SLC4A1 antibody (PB9437) .
Band 3/AE1/SLC4A1 was detected in a paraffin-embedded section of human kidney tissue. The tissue section was incubated with rabbit anti-Band 3/AE1/SLC4A1 Antibody (PB9437) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(9)
IF analysis of Band 3 using anti-Band 3 antibody (PB9437)
Band 3 was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-Band 3 Antibody (PB9437) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
all(9)
IF analysis of Band 3 using anti-Band 3 antibody (PB9437)
Band 3 was detected in paraffin-embedded section of rat kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-Band 3 Antibody (PB9437) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Flow Cytometry analysis of K562 cells using anti-Band 3/AE1/SLC4A1 antibody (PB9437).
Overlay histogram showing K562 cells stained with PB9437 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Band 3/AE1/SLC4A1 Antibody (PB9437) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-Band 3/AE1/SLC4A1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

AE 1; AE1; Anion exchange protein 1; Anion exchanger 1; band 3; Band 3 anion transport protein; band 3/ AE1; CD233; SLC4A1

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, IF, FCM

基因名称

SLC4A1

宿主

Rabbit

抗体亚型

IgG

免疫原

E. coli-derived human Band 3 (Position: E28-N365). Human Band 3 shares 75.7% and 74.5% amino acid (aa) sequence identity with mouse and rat Band 3, respectively.

计算分子量

102 kDa

实际分子量

102 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Band 3 is also known as SLC4A1. The protein encoded by this gene is part of the anion exchanger (AE) family and is expressed in the erythrocyte plasma membrane, where it functions as a chloride/bicarbonate exchanger involved in carbon dioxide transport from tissues to lungs. The protein comprises two domains that are structurally and functionally distinct. The N-terminal 40kDa domain is located in the cytoplasm and acts as an attachment site for the red cell skeleton by binding ankyrin. The glycosylated C-terminal membrane-associated domain contains 12-14 membrane spanning segments and carries out the stilbene disulphonate-sensitive exchange transport of anions. The cytoplasmic tail at the extreme C-terminus of the membrane domain binds carbonic anhydrase II. The encoded protein associates with the red cell membrane protein glycophorin A and this association promotes the correct folding and translocation of the exchanger. This protein is predominantly dimeric but forms tetramers in the presence of ankyrin. Many mutations in this gene are known in man, and these mutations can lead to two types of disease: destabilization of red cell membrane leading to hereditary spherocytosis, and defective kidney acid secretion leading to distal renal tubular acidosis. Other mutations that do not give rise to disease result in novel blood group antigens, which form the Diego blood group system.

Uniprot ID

P02730

RRID

AB_3082114

文献引用格式

Band 3/AE1/SLC4A1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB9437)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

solute carrier family 4 (anion exchanger), member 1 (Diego blood group)

蛋白名全称

Band 3 anion transport protein

实验方案>

实验步骤

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