Anti-VDAC1 Antibody

Rabbit Polyclonal Antibody

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筛选器： All WB IHC

PB9455

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of VDAC1 using anti-VDAC1 antibody (PB9455). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Caco-2 whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: rat heart tissue lysates,
Lane 6: rat kidney tissue lysates,
Lane 7: mouse heart tissue lysates,
Lane 8: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-VDAC1 antigen affinity purified polyclonal antibody (PB9455) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for VDAC1 at approximately 31 kDa. The expected band size for VDAC1 is at 31 kDa.
all(4)
IHC analysis of VDAC1 using anti-VDAC1 antibody (PB9455).
VDAC1 was detected in a paraffin-embedded section of mouse cardiac muscle tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-VDAC1 Antibody (PB9455) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(4)
IHC analysis of VDAC1 using anti-VDAC1 antibody (PB9455).
VDAC1 was detected in a paraffin-embedded section of rat cardiac muscle tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-VDAC1 Antibody (PB9455) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(4)
IHC analysis of VDAC1 using anti-VDAC1 antibody (PB9455).
VDAC1 was detected in a paraffin-embedded section of human intestinal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-VDAC1 Antibody (PB9455) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(4)

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产品简介>

产品名称

Anti-VDAC1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

hVDAC1; Plasmalemmal porin; Porin; PORIN 31 HL; Porin 31HL; Porin 31HM; VDAC; VDAC 1; VDAC1; VDAC2

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC

基因名称

VDAC1

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence in the middle region of human VDAC/Porin, different from the related mouse and rat sequences by one amino acid.

计算分子量

31 kDa

实际分子量

31 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

The voltage-dependent anion channel (VDAC) of the outer mitochondrial membrane is a small, abundant outer membrane pore-forming protein found in the outer membranes of all eukaryotic mitochondria. The VDAC protein is thought to form the major pathway for movement of adenine nucleotides through the outer membrane and to be the mitochondrial binding site for hexokinase and glycerol kinase. At low transmembrane voltage, VDAC is open for anions such as phosphate, chloride, and adenine nucleotides. At higher transmembrane voltage, VDAC functions as a selective channel for cations and uncharged molecules. These features make VDAC likely to play a role in mitochondrial energy metabolism. Huizing et al. studied by Northern and Western blot analyses the human tissue distribution of mitochondrial transmembrane metabolite carriers. They found that VDAC1 mRNA has a ubiquitous distribution, with most pronounced expression in heart, liver, and skeletal muscle, whereas the VDAC2 isoform appears to be expressed only in the heart.

Uniprot ID

P21796

RRID

AB_3082122

文献引用格式

VDAC1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB9455)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

voltage-dependent anion channel 1

实验方案>

实验步骤

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