Anti-CDC42 Antibody

筛选器： All WB ICC/IF FCM ELISA

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  Western blot analysis of anti-CDC42 antibody (A00119-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Hela whole cell lysates,
  Lane 2: human Jurkat whole cell lysates,
  Lane 3: human A431 whole cell lysates,
  Lane 4: human 293T whole cell lysates,
  Lane 5: rat brain tissue lysates,
  Lane 6: rat C6 whole cell lysates,
  Lane 7: mouse brain tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CDC42 antigen affinity purified polyclonal antibody (A00119-1) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CDC42 at approximately 21 kDa. The expected band size for CDC42 is at 21 kDa.

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  ICC/IF analysis of CDC42 using anti-CDC42 antibody (A00119-1).
  CDC42 was detected in an immunocytochemical section of U2OS cells. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

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  Flow Cytometry analysis of MCF-7 cells using anti-CDC42 antibody (A00119-1).
  Overlay histogram showing MCF-7 cells stained with A00119-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CDC42 Antibody (A00119-1, 1:100). Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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  Western blot analysis of anti-CDC42 antibody (A00119-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Hela whole cell lysates,
  Lane 2: human Jurkat whole cell lysates,
  Lane 3: human A431 whole cell lysates,
  Lane 4: human 293T whole cell lysates,
  Lane 5: rat brain tissue lysates,
  Lane 6: rat C6 whole cell lysates,
  Lane 7: mouse brain tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CDC42 antigen affinity purified polyclonal antibody (A00119-1) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CDC42 at approximately 21 kDa. The expected band size for CDC42 is at 21 kDa.

• 

  ICC/IF analysis of CDC42 using anti-CDC42 antibody (A00119-1).
  CDC42 was detected in an immunocytochemical section of U2OS cells. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

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  Flow Cytometry analysis of MCF-7 cells using anti-CDC42 antibody (A00119-1).
  Overlay histogram showing MCF-7 cells stained with A00119-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CDC42 Antibody (A00119-1, 1:100). Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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