Anti-CDC42 Antibody (Clone#IAE-3)

Rabbit monoclonal antibody

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筛选器： All WB IHC IP FCM

BM4787

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti- Anti-CDC42 Antibody(BM4787). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Jurkat whole cell lysates,
Lane 2: HELA whole cell lysates,
Lane 3:HepG2 whole cell lysates,
Lane 4: mouse spleen tissue lysates,
Lane 5: NIH3T3 whole cell lysates.
Use rabbit anti- CDC42 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for CDC42 at approximately 21KD. The expected band size for CDC42 is at 21KD.
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产品简介>

产品名称

Anti-CDC42 Antibody (Clone#IAE-3)

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

CDC42; CDC42Hs; G25K; G25K GTP binding protein

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, IP, FCM

基因名称

CDC42

克隆号

IAE-3

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human CDC42

计算分子量

21 kDa

实际分子量

21 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Cell division control protein 42 homolog also known as CDC42 is a protein involved in regulation of the cell cycle. In humans, CDC42 is encoded by the CDC42 gene.CDC42 is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants.

Uniprot ID

P60953

文献引用格式

CDC42 Antibody (Clone#IAE-3) (Boster Biological Technology, Wuhan, China. Catalog#BM4787)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Cell division control protein 42 homolog

实验方案>

实验步骤

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