Anti-ICAM1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC ELISA

A00171-1

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of ICAM1 using anti-ICAM1 antibody (A00171-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Mouse spleen tissue lysates,
Lane 2: mouse thymus tissue lysates,
Lane 3: Mouse liver tissue lysates,
Lane 4: Mouse kidney tissue lysates,
Lane 5: Mouse lung tissue lysates,
Lane 6: Mouse RAW264.7 whole cell lysates,
Lane 7: Mouse ANA-1 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ICAM1 antigen affinity purified polyclonal antibody (A00171-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ICAM1 at approximately 90-110 kDa. The expected band size for ICAM1 is at 59 kDa.
all(3)
IHC analysis of ICAM1 using anti-ICAM1 antibody (A00171-1).
ICAM1 was detected in a paraffin-embedded section of mouse intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ICAM1 Antibody (A00171-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(3)
all(3)

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产品简介>

产品名称

Anti-ICAM1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

BB2; CD54; ICAM 1; ICAM1; ICAM-1; P3.58; Ly 47; MALA 2

产品类型

Polyclonal

检验物种

mouse

应用范围

WB, IHC, ELISA

基因名称

Icam1

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived mouse Icam1 recombinant protein (Position: K53-P537).

计算分子量

59 kDa

实际分子量

90-110 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

CD54, also known as ICAM-1. Intercellular adhesion molecule-1 (ICAM1) is a ligand for lymphocyte function-associated (LFA) antigens. ICAM-1 is an integral membrane protein, a member of the immunoglobulin superfamily, and a ligand for LFA-1, a beta 2 leukocyte integrin. This protein is the major human rhinovirus receptor. The ICAM1 gene is mapped to human chromosome 19. In humans, lymphocyte adhesion to cells is mediated by the protein heterodimer CD11a/CD18 (Leu-CAMa, LFA-1) and its ligand CD54 (ICAM-1).

Uniprot ID

P13597

RRID

AB_3080994

文献引用格式

ICAM1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00171-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

intercellular adhesion molecule 1

蛋白名全称

Intercellular adhesion molecule 1

实验方案>

实验步骤

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