Anti-ICAM1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC

PB9018

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of ICAM1 using anti-ICAM1 antibody (PB9018). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat NRK whole cell lysates,
Lane 2: mouse spleen tissue lysates,
Lane 3: mouse liver tissue lysates,
Lane 4: mouse kidney tissue lysates,
Lane 5: mouse ANA-1 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ICAM1 antigen affinity purified polyclonal antibody (PB9018) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ICAM1 at approximately 90-110 kDa. The expected band size for ICAM1 is at 59 kDa.
all(5)
IHC analysis of ICAM1 using anti-ICAM1 antibody (PB9018).
ICAM1 was detected in a paraffin-embedded section of mouse liver tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ICAM1 Antibody (PB9018) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(5)
IHC analysis of ICAM1 using anti-ICAM1 antibody (PB9018).
ICAM1 was detected in a paraffin-embedded section of mouse lung tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ICAM1 Antibody (PB9018) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(5)
IHC analysis of ICAM1 using anti-ICAM1 antibody (PB9018).
ICAM1 was detected in a paraffin-embedded section of rat lung tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ICAM1 Antibody (PB9018) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(5)
IHC analysis of ICAM1 using anti-ICAM1 antibody (PB9018).
ICAM1 was detected in a paraffin-embedded section of rat liver tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ICAM1 Antibody (PB9018) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(5)

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产品简介>

产品名称

Anti-ICAM1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

BB2; CD54; ICAM 1; ICAM1; ICAM-1; P3.58; Ly 47; MALA 2

产品类型

Polyclonal

检验物种

mouse, rat

应用范围

WB, IHC

基因名称

ICAM1

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived mouse ICAM1 recombinant protein (Position: G198-P537). Mouse ICAM1 shares 74% amino acid (aa) sequence identity with rat ICAM1.

计算分子量

59 kDa

实际分子量

90-110 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Intercellular adhesion molecule-1 (ICAM-1) is an integral membrane protein, a member of the immunoglobulin superfamily, and a ligand for lymphocyte function-associated (LFA) antigens, a beta 2 leukocyte integrin. The normal function of human ICAM-1 is to provide adhesion between endothelial cells and leukocytes after injury or stress. ICAM-1 binds to leukocyte function-associated antigen (LFA-1) or macrophage-1 antigen (Mac-1). It is found on leukocytes, fibroblasts, epithelial cells and endothelial cells and its expression is regulated by inflammatory cytokines. ICAM-1 has a tissue distribution similar to that of the major histocompatibility complex class II antigens and is likely to play a role in inflammatory responses.

Uniprot ID

P13597

RRID

AB_3081925

文献引用格式

ICAM1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB9018)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

intercellular adhesion molecule 1

蛋白名全称

Intercellular adhesion molecule 1

实验方案>

实验步骤

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