Anti-Bcl-XL/BCL2L1 Antibody

筛选器： All WB FCM

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  Western blot analysis of Bcl-XL/BCL2L1 using anti-Bcl-XL/BCL2L1 antibody (A00181). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Jurkat whole cell lysates,
  Lane 2: human Hela whole cell lysates,
  Lane 3: human A549 whole cell lysates,
  Lane 4: human Raji whole cell lysates,
  Lane 5: rat brain tissue lysates,
  Lane 6: rat C6 whole cell lysates,
  Lane 7: mouse brain tissue lysates,
  Lane 8: mouse NIH/3T3 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Bcl-XL/BCL2L1 antigen affinity purified polyclonal antibody (A00181) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Bcl-XL/BCL2L1 at approximately 26-30 kDa. The expected band size for Bcl-XL/BCL2L1 is at 26 kDa.

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  Flow Cytometry analysis of Jurkat cells using anti-Bcl-XL/BCL2L1 antibody (A00181).
  Overlay histogram showing Jurkat cells stained with A00181 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Bcl-XL/BCL2L1 Antibody (A00181) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample (Red line) was also used as a control.

• 

  Western blot analysis of Bcl-XL/BCL2L1 using anti-Bcl-XL/BCL2L1 antibody (A00181). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Jurkat whole cell lysates,
  Lane 2: human Hela whole cell lysates,
  Lane 3: human A549 whole cell lysates,
  Lane 4: human Raji whole cell lysates,
  Lane 5: rat brain tissue lysates,
  Lane 6: rat C6 whole cell lysates,
  Lane 7: mouse brain tissue lysates,
  Lane 8: mouse NIH/3T3 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Bcl-XL/BCL2L1 antigen affinity purified polyclonal antibody (A00181) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Bcl-XL/BCL2L1 at approximately 26-30 kDa. The expected band size for Bcl-XL/BCL2L1 is at 26 kDa.

• 

  Flow Cytometry analysis of Jurkat cells using anti-Bcl-XL/BCL2L1 antibody (A00181).
  Overlay histogram showing Jurkat cells stained with A00181 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Bcl-XL/BCL2L1 Antibody (A00181) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample (Red line) was also used as a control.