Western blot analysis of Bcl-X/BCL2L1 using anti-Bcl-X/BCL2L1 antibody (PB9917). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: SW620 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Bcl-X/BCL2L1 antigen affinity purified polyclonal antibody (PB9917) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Bcl-X/BCL2L1 at approximately 26 kDa、19 kDa. The expected band size for Bcl-X/BCL2L1 is at 26 kDa.
Flow Cytometry analysis of PC-3 cells using anti-Bcl-X/BCL2L1 antibody (PB9917).
Overlay histogram showing PC-3 cells stained with PB9917 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Bcl-X/BCL2L1 Antibody (PB9917) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Flow Cytometry analysis of A549 cells using anti-Bcl-X/BCL2L1 antibody (PB9917).
Overlay histogram showing A549 cells stained with PB9917 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Bcl-X/BCL2L1 Antibody (PB9917) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
| Western blot (WB): | 1:500-2000 |
| Flow Cytometry (Fixed): | 1:50-200 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Western blot analysis of Bcl-X/BCL2L1 using anti-Bcl-X/BCL2L1 antibody (PB9917). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: SW620 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Bcl-X/BCL2L1 antigen affinity purified polyclonal antibody (PB9917) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Bcl-X/BCL2L1 at approximately 26 kDa、19 kDa. The expected band size for Bcl-X/BCL2L1 is at 26 kDa.
Flow Cytometry analysis of PC-3 cells using anti-Bcl-X/BCL2L1 antibody (PB9917).
Overlay histogram showing PC-3 cells stained with PB9917 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Bcl-X/BCL2L1 Antibody (PB9917) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Flow Cytometry analysis of A549 cells using anti-Bcl-X/BCL2L1 antibody (PB9917).
Overlay histogram showing A549 cells stained with PB9917 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Bcl-X/BCL2L1 Antibody (PB9917) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Western blot analysis of Bcl-X/BCL2L1 using anti-Bcl-X/BCL2L1 antibody (PB9917). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: SW620 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Bcl-X/BCL2L1 antigen affinity purified polyclonal antibody (PB9917) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Bcl-X/BCL2L1 at approximately 26 kDa、19 kDa. The expected band size for Bcl-X/BCL2L1 is at 26 kDa.
Flow Cytometry analysis of PC-3 cells using anti-Bcl-X/BCL2L1 antibody (PB9917).
Overlay histogram showing PC-3 cells stained with PB9917 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Bcl-X/BCL2L1 Antibody (PB9917) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Flow Cytometry analysis of A549 cells using anti-Bcl-X/BCL2L1 antibody (PB9917).
Overlay histogram showing A549 cells stained with PB9917 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Bcl-X/BCL2L1 Antibody (PB9917) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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