Anti-Bcl-XL/BCL2L1 Antibody (Clone#AOD-2)

Rabbit monoclonal antibody

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筛选器： All WB IHC ICC/IF IP FCM

BM3956

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Western blot analysis of anti-Bcl-XL/BCL2L1 antibody (BM3956). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human SH-SY5Y whole cell lysates,
Lane 5: rat spleen tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse spleen tissue lysates,
Lane 8: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Bcl-XL/BCL2L1 antigen affinity purified monoclonal antibody (BM3956) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Bcl-XL/BCL2L1 at approximately 30 kDa. The expected band size for Bcl-XL/BCL2L1 is at 26 kDa.
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Immunohistochemical analysis of paraffin-embedded Human pituitary tumor, using the Antibody.
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Immunohistochemical analysis of paraffin-embedded Human liver cancer, using the Antibody.
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Immunohistochemical analysis of paraffin-embedded human liver cancer, using Bcl-XL Antibody.
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Immunofluorescent analysis using the Antibody.
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Immunofluorescent analysis of Hela cells, using Bcl-XL Antibody .
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产品简介>

产品名称

Anti-Bcl-XL/BCL2L1 Antibody (Clone#AOD-2)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Apoptosis regulator Bcl X; BCL2L1; Bcl-XL

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, IP, FCM

基因名称

BCL2L1

克隆号

AOD-2

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human Bcl-XL

计算分子量

26 kDa

实际分子量

26 kDa、19 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Bcl-2-like protein 1 is a protein that in humans is encoded by the BCL2L1 gene. The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Alternative splicing results in multiple transcript variants encoding two different isoforms. The longer isoform (Bcl-xL) acts as an apoptotic inhibitor and the shorter form (Bcl-xS) acts as an apoptotic activator.

Uniprot ID

Q07817

文献引用格式

Bcl-XL/BCL2L1 Antibody (Clone#AOD-2) (Boster Biological Technology, Wuhan, China. Catalog#BM3956)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Bcl-2-like protein 1

实验方案>

实验步骤

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