Anti-Bcl-XL/BCL2L1 Antibody (Clone#OTI4A9)

Mouse monoclonal antibody

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筛选器： All WB IHC ICC/IF

M00181-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Western blot analysis of anti-BCL-XL antibody (M00181-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-BCL-XL antigen affinity purified monoclonal antibody (M00181-2) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for BCL-XL at approximately 30 kDa. The expected band size for BCL-XL is at 26 kDa.
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-BCL-XL mouse monoclonal antibody. (M00181-2) (1:150)
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Immunofluorescent staining of HT29 cells using anti-BCL2L1 mouse monoclonal antibody (1:50).
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-BCL-XL mouse monoclonal antibody. (M00181-2) (1:150)
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Anti-BCL2L1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BCL2L1 (1:100).
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产品简介>

产品名称

Anti-Bcl-XL/BCL2L1 Antibody (Clone#OTI4A9)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Apoptosis regulator Bcl X; BCL2L1; Bcl-XL

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF

基因名称

BCL2L1

克隆号

OTI4A9

宿主

Mouse

抗体亚型

IgG2a

免疫原

Recombinant fragment expressed in E.coli corresponding to amino acids 1-212 of human Bcl-XL

计算分子量

26 kDa

实际分子量

25.9 kDa

成分

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

0.25 mg/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

Bcl-2-like protein 1, also known as Bcl-XL, is a protein that in humans is encoded by the BCL2L1 gene. The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Alternative splicing results in multiple transcript variants encoding two different isoforms. The longer isoform (Bcl-xL) acts as an apoptotic inhibitor and the shorter form (Bcl-xS) acts as an apoptotic activator.

Uniprot ID

Q07817

文献引用格式

Bcl-XL/BCL2L1 Antibody (Clone#OTI4A9) (Boster Biological Technology, Wuhan, China. Catalog#M00181-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

BCL2-like 1

蛋白名全称

Bcl-2-like protein 1(Bcl2-L-1)

实验方案>

实验步骤

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