Anti-FGFR2 Antibody

筛选器： All WB FCM ELISA

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  Western blot analysis of anti- FGFR2 antibody (A00231-3). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
  Lane 1: human MCF-7 whole cell lysates,
  Lane 2: human Hela whole cell lysates,
  Lane 3: human K562 whole cell lysates.
  Use rabbit anti- FGFR2 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for FGFR2 at approximately 135KD. The expected band size for FGFR2 is at 92KD.

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  Flow Cytometry analysis of U2OS cells using anti-FGFR2 antibody (A00231-3).
  Overlay histogram showing U2OS cells stained with A00231-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-FGFR2 Antibody (A00231-3) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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  Western blot analysis of anti- FGFR2 antibody (A00231-3). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
  Lane 1: human MCF-7 whole cell lysates,
  Lane 2: human Hela whole cell lysates,
  Lane 3: human K562 whole cell lysates.
  Use rabbit anti- FGFR2 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for FGFR2 at approximately 135KD. The expected band size for FGFR2 is at 92KD.

• 

  Flow Cytometry analysis of U2OS cells using anti-FGFR2 antibody (A00231-3).
  Overlay histogram showing U2OS cells stained with A00231-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-FGFR2 Antibody (A00231-3) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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