Anti-FGFR2 Antibody (Clone#OTI1D3)

Mouse monoclonal antibody

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筛选器： All WB FCM

M00231-3

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Cross-Reactivity of anti-FGFR2 (M00231-3) with FGFR1 and FGFR3, not with FGFR4.HEK293T cells were transfected with the DDK (FLAG)-tagged DNAs of FGFR2 , FGFR1 , FGFR3 , FGFR4 cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FGFR2 (M00231-3). (1:500) and anti-DDK (1:2000), respectively.
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Western blot analysis of extracts (10ug) from 8 Human tissue by using anti-FGFR2 monoclonal antibody at 1:200 (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: Colon).
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Flow cytometric Analysis of Hela cells, using anti-FGFR2 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
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Flow cytometric Analysis of Jurkat cells, using anti-FGFR2 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
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Western blot analysis of extracts (35ug) from 2 different cell by using anti-FGFR2 monoclonal antibody (1:250).
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FGFR2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FGFR2 (1:500).
all(6)

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产品简介>

产品名称

Anti-FGFR2 Antibody (Clone#OTI1D3)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

BEK; BFR 1; CD332; CEK3; CFD1; ECT1; FGFR 2; FGFR2; JWS; K SAM; KGFR; KSAM; TK14; TK25

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, FCM

基因名称

FGFR2

克隆号

OTI1D3

宿主

Mouse

抗体亚型

IgG2b

免疫原

Full length human recombinant protein of human FGFR2 (NP_000132) produced in HEK293T cell.

计算分子量

92 kDa

实际分子量

89.7 kDa

成分

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

The protein encoded by this gene is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member is a high-affinity receptor for acidic, basic and/or keratinocyte growth factor, depending on the isoform. Mutations in this gene are associated with Crouzon syndrome, Pfeiffer syndrome, Craniosynostosis, Apert syndrome, Jackson-Weiss syndrome, Beare-Stevenson cutis gyrata syndrome, Saethre-Chotzen syndrome, and syndromic craniosynostosis. Multiple alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jan 2009]

Uniprot ID

P21802

文献引用格式

FGFR2 Antibody (Clone#OTI1D3) (Boster Biological Technology, Wuhan, China. Catalog#M00231-3)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

fibroblast growth factor receptor 2

蛋白名全称

Fibroblast growth factor receptor 2

实验方案>

实验步骤

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