| Western blot (WB): | 1:500-2000 |
| ELISA(Cap): | 1:50-1:200 |

Western blot analysis of IGFBP3 using anti-IGFBP3 antibody (A00435-2).
Lane 1: recombinant human IGFBP3 protein 1ng.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-IGFBP3 antigen affinity purified polyclonal antibody (A00435-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for IGFBP3 at approximately 37 kDa.

Western blot analysis of IGFBP3 using anti-IGFBP3 antibody (A00435-2).
Lane 1: recombinant human IGFBP3 protein 1ng.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-IGFBP3 antigen affinity purified polyclonal antibody (A00435-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for IGFBP3 at approximately 37 kDa.


