| Western blot (WB): | 1:500-2000 |
| ELISA: | 1:100-1000 |

Western blot analysis of IGFBP3 using anti-IGFBP3 antibody (A00435-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-IGFBP3 antigen affinity purified polyclonal antibody (A00435-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for IGFBP3 at approximately 32 kDa. The expected band size for IGFBP3 is at 32 kDa.

Western blot analysis of IGFBP3 using anti-IGFBP3 antibody (A00435-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-IGFBP3 antigen affinity purified polyclonal antibody (A00435-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for IGFBP3 at approximately 32 kDa. The expected band size for IGFBP3 is at 32 kDa.


