| Western blot (WB): | 1:500-2000 |

Western blot analysis of IGFBP3 using anti-IGFBP3 antibody (BA3641). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: 293T whole cell lysates,
Lane 2: MCF-7 whole cell lysates,
Lane 3: A549 whole cell lysates,
Lane 4: SW620 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-IGFBP3 antigen affinity purified polyclonal antibody (BA3641) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for IGFBP3 at approximately 32 kDa. The expected band size for IGFBP3 is at 32 kDa.

Western blot analysis of IGFBP3 using anti-IGFBP3 antibody (BA3641). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat liver tissue lysates,
Lane 2: Rat kidney tissue lysates,
Lane 3: Rat heart tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-IGFBP3 antigen affinity purified polyclonal antibody (BA3641) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for IGFBP3 at approximately 32 kDa. The expected band size for IGFBP3 is at 32 kDa.

Western blot analysis of IGFBP3 using anti-IGFBP3 antibody (BA3641). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: 293T whole cell lysates,
Lane 2: MCF-7 whole cell lysates,
Lane 3: A549 whole cell lysates,
Lane 4: SW620 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-IGFBP3 antigen affinity purified polyclonal antibody (BA3641) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for IGFBP3 at approximately 32 kDa. The expected band size for IGFBP3 is at 32 kDa.

Western blot analysis of IGFBP3 using anti-IGFBP3 antibody (BA3641). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat liver tissue lysates,
Lane 2: Rat kidney tissue lysates,
Lane 3: Rat heart tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-IGFBP3 antigen affinity purified polyclonal antibody (BA3641) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for IGFBP3 at approximately 32 kDa. The expected band size for IGFBP3 is at 32 kDa.

