Anti-ABCC2 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC

A00974-1

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Figure 1. Western blot analysis of ABCC2 using anti-ABCC2 antibody (A00974-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ABCC2 antigen affinity purified polyclonal antibody (A00974-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ABCC2 at approximately 170-250 kDa. The expected band size for ABCC2 is at 174 kDa.
all(3)
Figure 2. IHC analysis of ABCC2 using anti-ABCC2 antibody (A00974-1).
ABCC2 was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-ABCC2 Antibody (A00974-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(3)
Figure 3. IHC analysis of ABCC2 using anti-ABCC2 antibody (A00974-1).
ABCC2 was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-ABCC2 Antibody (A00974-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(3)

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产品简介>

产品名称

Anti-ABCC2 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

ABC30; ABCC2; CMOAT; CMOAT1; cMRP; DJS; KIAA1010; MRP2

产品类型

Polyclonal

检验物种

human

应用范围

WB, IHC

基因名称

ABCC2

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence in the middle region of human MRP2/ABCC2, which shares 77.1% and 71.4% amino acid (aa) sequence identity with mouse and rat MRP2/ABCC2, respectively.

计算分子量

174 kDa

实际分子量

170-250 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Multidrug resistance-associated protein 2 (MRP2), also called canalicular multispecific organic anion transporter 1 (cMOAT) or ATP-binding cassette sub-family C member 2 (ABCC2), is a protein that in humans is encoded by the ABCC2 gene. The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This protein is expressed in the canalicular (apical) part of the hepatocyte and functions in biliary transport. Substrates include anticancer drugs such as vinblastine; therefore, this protein appears to contribute to drug resistance in mammalian cells. Several different mutations in this gene have been observed in patients with Dubin-Johnson syndrome (DJS), an autosomal recessive disorder characterized by conjugated hyperbilirubinemia.

Uniprot ID

Q92887

文献引用格式

ABCC2 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00974-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

实验方案>

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