Anti-VCAM1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC ELISA(Cap)

A01199

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of VCAM1 using anti-VCAM1 antibody (A01199). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse spleen tissue lysates,
Lane 2: mouse lung tissue lysates,
Lane 3: rat spleen tissue lysates,
Lane 4: rat lung tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-VCAM1 antigen affinity purified polyclonal antibody (A01199) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for VCAM1 at approximately 85-110 kDa. The expected band size for VCAM1 is at 81 kDa.
all(4)
IHC analysis of VCAM1 using anti-VCAM1 antibody (A01199).
VCAM1 was detected in a paraffin-embedded section of mouse spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-VCAM1 Antibody (A01199) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(4)
IHC analysis of VCAM1 using anti-VCAM1 antibody (A01199).
VCAM1 was detected in a paraffin-embedded section of rat spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-VCAM1 Antibody (A01199) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(4)
all(4)

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产品简介>

产品名称

Anti-VCAM1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

CD106; INCAM 100; V CAM 1; VCAM 1; VCAM1

产品类型

Polyclonal

检验物种

mouse, rat

应用范围

WB, IHC, ELISA(Cap)

基因名称

Vcam1

宿主

Rabbit

抗体亚型

IgG

免疫原

E. coli-derived mouse VCAM1 recombinant protein (Position: F25-L270).

计算分子量

81 kDa

实际分子量

85-110 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

CD106(cluster of differentiation 106) also known as vascular cell adhesion molecule 1(VCAM-1), is a protein that in humans is encoded by the VCAM1 gene. In inflammatory conditions and in cardiac allografts undergoing rejection, VCAM1 is upregulated in endothelium of postcapillary venules. Arterial expression of VCAM1 is also found in experimental models of atherosclerosis in the rabbit. This gene is mapped to chromosome 1 by Southern analysis of somatic cell hybrids. VCAM-1 functions as a cell adhesion molecule. The VCAM-1 protein mediates the adhesion of lymphocytes, monocytes, eosinophils, andbasophils to vascularendothelium. It also functions in leukocyte-endothelial cell signal transduction, and it may play a role in the development ofatherosclerosis and rheumatoid arthritis. CAM741 works similar to cotransin in that it represses the biosynthesis of VCAM1 cells by blocking the process of cotranslational translocation, which is dependent on the signal peptide ofVCAM1. Among the lung metastasis signature genes identified, several, includingVCAM1, were functionally validated.

Uniprot ID

P29533

RRID

AB_3081375

文献引用格式

VCAM1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A01199)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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