| Western blot (WB): | 1:500~2000 |
| Immunohistochemistry (IHC): | 1:150 |

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-VCAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01199-2)

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-VCAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01199-2)

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-VCAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01199-2)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-VCAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01199-2)

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-VCAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01199-2)

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-VCAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01199-2)

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-VCAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01199-2)

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-VCAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01199-2)

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-VCAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01199-2)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-VCAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01199-2)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VCAM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VCAM1.

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-VCAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01199-2)

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-VCAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01199-2)

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-VCAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01199-2)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-VCAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01199-2)

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-VCAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01199-2)

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-VCAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01199-2)

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-VCAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01199-2)

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-VCAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01199-2)

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-VCAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01199-2)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-VCAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01199-2)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VCAM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VCAM1.










