Anti-CD31/PECAM1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC ELISA

A01513-3

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of CD31/PECAM1 using anti-CD31/PECAM1 antibody (A01513-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: rat lung tissue lysates,
Lane 3: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD31/PECAM1 antigen affinity purified polyclonal antibody (A01513-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD31/PECAM1 at approximately 120-130 kDa. The expected band size for CD31/PECAM1 is at 76 kDa.
all(5)
IHC analysis of CD31/PECAM1 using anti-CD31/PECAM1 antibody (A01513-3).
CD31/PECAM1 was detected in a paraffin-embedded section of human placenta tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD31/PECAM1 Antibody (A01513-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(5)
IHC analysis of CD31/PECAM1 using anti-CD31/PECAM1 antibody (A01513-3).
CD31/PECAM1 was detected in a paraffin-embedded section of mouse lung tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD31/PECAM1 Antibody (A01513-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(5)
IHC analysis of CD31/PECAM1 using anti-CD31/PECAM1 antibody (A01513-3).
CD31/PECAM1 was detected in a paraffin-embedded section of rat liver tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD31/PECAM1 Antibody (A01513-3) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(5)
all(5)

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产品简介>

产品名称

Anti-CD31/PECAM1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

CD31; EndoCAM; GPIIA; PECA1; PECAM 1; PECAM1; VEC marker

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ELISA

基因名称

Pecam1

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived rat CD31/Pecam1 recombinant protein (Position: Q41-E491).

计算分子量

76 kDa

实际分子量

120-130 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

CD31 also known as Platelet endothelial cell adhesion molecule (PECAM-1),is a protein that in human is encoded by the PECAM1 gene. Encoded protein is a member of the immunoglobulin superfamily and this gene is mapped to 17q23.3. CD31 is found on the surface of platelets,monocytes,neutrophils,and some types of T-cells,and makes up a large portion of endothelial cell intercellular junctions. It is demonstrated that CD31 expression on human PBSCs may positively affect both neutrophil and platelet engraftment. Meanwhile,CD31 is involved in leukocyte migration and angiogenesis,which are key components of venous thrombus resolution.

Uniprot ID

Q3SWT0

RRID

AB_3081452

文献引用格式

CD31/PECAM1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A01513-3)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

platelet and endothelial cell adhesion molecule 1

蛋白名全称

Platelet endothelial cell adhesion molecule

实验方案>

实验步骤

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