Western blot (WB): | 1:500-2000 |
Immunohistochemistry (IHC): | 1:50-400 |
Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |
Immunohistochemistry (IHC): | 1:50-400 |
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
Western blot analysis of CD31/PECAM1 using anti-CD31/PECAM1 antibody (A01513-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat lung tissue lysates,
Lane 2: rat spleen tissue lysates,
Lane 3: rat heart tissue lysates,
Lane 4: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD31/PECAM1 antigA03957-Aen affinity purified polyclonal antibody (A01513-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD31/PECAM1 at approximately 100-120 kDa. The expected band size for CD31/PECAM1 is at 83 kDa.
IHC analysis of CD31/PECAM1 using anti-CD31/PECAM1 antibody (A01513-2).
CD31/PECAM1 was detected in a paraffin-embedded section of mouse colon tissue. The tissue section was incubated with rabbit anti-CD31/PECAM1 Antibody (A01513-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of CD31/PECAM1 using anti-CD31/PECAM1 antibody (A01513-2).
CD31/PECAM1 was detected in a paraffin-embedded section of mouse kidney tissue. The tissue section was incubated with rabbit anti-CD31/PECAM1 Antibody (A01513-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of CD31/PECAM1 using anti-CD31/PECAM1 antibody (A01513-2).
CD31/PECAM1 was detected in a paraffin-embedded section of mouse kidney tissue. The tissue section was incubated with rabbit anti-CD31/PECAM1 Antibody (A01513-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of CD31/PECAM1 using anti-CD31/PECAM1 antibody (A01513-2).
CD31/PECAM1 was detected in a paraffin-embedded section of rat colon tissue. The tissue section was incubated with rabbit anti-CD31/PECAM1 Antibody (A01513-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of CD31/PECAM1 using anti-CD31/PECAM1 antibody (A01513-2).
CD31/PECAM1 was detected in a paraffin-embedded section of rat heart tissue. The tissue section was incubated with rabbit anti-CD31/PECAM1 Antibody (A01513-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of CD31/PECAM1 using anti-CD31/PECAM1 antibody (A01513-2).
CD31/PECAM1 was detected in a paraffin-embedded section of rat kidney tissue. The tissue section was incubated with rabbit anti-CD31/PECAM1 Antibody (A01513-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of CD31/PECAM1 using anti-CD31/PECAM1 antibody (A01513-2).
CD31/PECAM1 was detected in a paraffin-embedded section of rat liver tissue. The tissue section was incubated with rabbit anti-CD31/PECAM1 Antibody (A01513-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of CD31/PECAM1 using anti-CD31/PECAM1 antibody (A01513-2).
CD31/PECAM1 was detected in a paraffin-embedded section of rat lung tissue. The tissue section was incubated with rabbit anti-CD31/PECAM1 Antibody (A01513-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
Western blot analysis of CD31/PECAM1 using anti-CD31/PECAM1 antibody (A01513-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat lung tissue lysates,
Lane 2: rat spleen tissue lysates,
Lane 3: rat heart tissue lysates,
Lane 4: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD31/PECAM1 antigA03957-Aen affinity purified polyclonal antibody (A01513-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD31/PECAM1 at approximately 100-120 kDa. The expected band size for CD31/PECAM1 is at 83 kDa.
IHC analysis of CD31/PECAM1 using anti-CD31/PECAM1 antibody (A01513-2).
CD31/PECAM1 was detected in a paraffin-embedded section of mouse colon tissue. The tissue section was incubated with rabbit anti-CD31/PECAM1 Antibody (A01513-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of CD31/PECAM1 using anti-CD31/PECAM1 antibody (A01513-2).
CD31/PECAM1 was detected in a paraffin-embedded section of mouse kidney tissue. The tissue section was incubated with rabbit anti-CD31/PECAM1 Antibody (A01513-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of CD31/PECAM1 using anti-CD31/PECAM1 antibody (A01513-2).
CD31/PECAM1 was detected in a paraffin-embedded section of mouse kidney tissue. The tissue section was incubated with rabbit anti-CD31/PECAM1 Antibody (A01513-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of CD31/PECAM1 using anti-CD31/PECAM1 antibody (A01513-2).
CD31/PECAM1 was detected in a paraffin-embedded section of rat colon tissue. The tissue section was incubated with rabbit anti-CD31/PECAM1 Antibody (A01513-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of CD31/PECAM1 using anti-CD31/PECAM1 antibody (A01513-2).
CD31/PECAM1 was detected in a paraffin-embedded section of rat heart tissue. The tissue section was incubated with rabbit anti-CD31/PECAM1 Antibody (A01513-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of CD31/PECAM1 using anti-CD31/PECAM1 antibody (A01513-2).
CD31/PECAM1 was detected in a paraffin-embedded section of rat kidney tissue. The tissue section was incubated with rabbit anti-CD31/PECAM1 Antibody (A01513-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of CD31/PECAM1 using anti-CD31/PECAM1 antibody (A01513-2).
CD31/PECAM1 was detected in a paraffin-embedded section of rat liver tissue. The tissue section was incubated with rabbit anti-CD31/PECAM1 Antibody (A01513-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of CD31/PECAM1 using anti-CD31/PECAM1 antibody (A01513-2).
CD31/PECAM1 was detected in a paraffin-embedded section of rat lung tissue. The tissue section was incubated with rabbit anti-CD31/PECAM1 Antibody (A01513-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.