Anti-CD31/PECAM1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC IF FCM

BA1346

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of CD31/PECAM1 using anti-CD31/PECAM1 antibody (BA1346). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEL whole cell lysates,
Lane 2: human Jurkat whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD31/PECAM1 antigen affinity purified polyclonal antibody (BA1346) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD31/PECAM1 at approximately 120 kDa. The expected band size for CD31/PECAM1 is at 83 kDa.
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IHC analysis of CD31/PECAM1 using anti-CD31/PECAM1 antibody (BA1346) .
CD31/PECAM1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. The tissue section was incubated with rabbit anti-CD31/PECAM1 Antibody (BA1346) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(9)
IHC analysis of CD31/PECAM1 using anti-CD31/PECAM1 antibody (BA1346) .
CD31/PECAM1 was detected in a paraffin-embedded section of human glioblastoma tissue. The tissue section was incubated with rabbit anti-CD31/PECAM1 Antibody (BA1346) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(9)
IHC analysis of CD31/PECAM1 using anti-CD31/PECAM1 antibody (BA1346) .
CD31/PECAM1 was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-CD31/PECAM1 Antibody (BA1346) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(9)
IHC analysis of CD31/PECAM1 using anti-CD31/PECAM1 antibody (BA1346) .
CD31/PECAM1 was detected in a paraffin-embedded section of human placenta tissue. The tissue section was incubated with rabbit anti-CD31/PECAM1 Antibody (BA1346) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(9)
IHC analysis of CD31/PECAM1 using anti-CD31/PECAM1 antibody (BA1346) .
CD31/PECAM1 was detected in a paraffin-embedded section of human testicular germ cell tumors tissue. The tissue section was incubated with rabbit anti-CD31/PECAM1 Antibody (BA1346) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(9)
IF analysis of CD31/PECAM1 using anti-CD31/PECAM1 antibody (BA1346).
CD31/PECAM1 was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-CD31/PECAM1 Antibody (BA1346) at a dilution of 1:100. Dylight594-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1142) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
all(9)
IF analysis of CD31/PECAM1 using anti-CD31/PECAM1 antibody (BA1346).
CD31/PECAM1 was detected in a paraffin-embedded section of human placenta tissue. The tissue section was incubated with rabbit anti-CD31/PECAM1 Antibody (BA1346) at a dilution of 1:100. Dylight594-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1142) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
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Flow Cytometry analysis of HEL cells using anti-CD31/PECAM1 antibody (BA1346).
Overlay histogram showing HEL cells stained with BA1346 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD31/PECAM1 Antibody (BA1346, 1:100). DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
all(9)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-CD31/PECAM1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

CD31; EndoCAM; GPIIA; PECA1; PECAM 1; PECAM1; VEC marker

产品类型

Polyclonal

检验物种

human

应用范围

WB, IHC, IF, FCM

基因名称

PECAM1

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of rat CD31.

计算分子量

83 kDa

实际分子量

120-130 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

PECAM-1(Platelet endothelial cell adhesion molecule), also known as cluster of differentiation 31(CD31) is a protein that in human is encoded by the PECAM1 gene found on chromosome 17. PECAM1 is a member of the immunoglobulin(Ig) superfamily that is expressed on the surface of circulating platelets, monocytes, neutrophils, and particular T-cell subsets. Using a PCR-based analysis of somatic cell hybrids, Gumina et al.(1996) mapped PECAM1 to chromosome 17 in the region 17q23-qter. By fluorescence in situ hybridization, they assigned the PECAM1 locus specifically to 17q23. Several adhesion molecules expressed on platelets and endothelium also localized to 17q. Xie and Muller(1996) mapped the Pecam1 gene to mouse chromosome 6, region F3-G1, by fluorescence in situ hybridization. PECAM-1 is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions, and PECAM-1 plays a key role in removing aged neutrophils from the body.

Uniprot ID

P16284

文献引用格式

CD31/PECAM1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA1346)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

platelet/endothelial cell adhesion molecule 1

蛋白名全称

Platelet endothelial cell adhesion molecule

实验方案>

实验步骤

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