Western blot analysis of Calbindin/CALB1 using anti-Calbindin/CALB1 antibody (A03047). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat kidney tissue lysates,
Lane 3: mouse brain tissue lysates,
Lane 4: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Calbindin/CALB1 antigen affinity purified polyclonal antibody (A03047) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Calbindin/CALB1 at approximately 28 kDa. The expected band size for Calbindin/CALB1 is at 30 kDa.
IHC analysis of Calbindin/CALB1 using anti-Calbindin/CALB1 antibody (A03047).
Calbindin/CALB1 was detected in a paraffin-embedded section of rat kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Calbindin/CALB1 Antibody (A03047) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Calbindin/CALB1 using anti-Calbindin/CALB1 antibody (A03047).
Calbindin/CALB1 was detected in a paraffin-embedded section of mouse brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Calbindin/CALB1 Antibody (A03047) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Calbindin/CALB1 using anti-Calbindin/CALB1 antibody (A03047).
Calbindin/CALB1 was detected in a paraffin-embedded section of mouse kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Calbindin/CALB1 Antibody (A03047) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Calbindin/CALB1 using anti-Calbindin/CALB1 antibody (A03047).
Calbindin/CALB1 was detected in a paraffin-embedded section of human cerebellum tissue. The tissue section was incubated with rabbit anti-Calbindin/CALB1 Antibody (A03047) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IF analysis using anti- Calbindin antibody (A03047). detected in paraffin-embedded section of rat cerebellum tissue. The tissue section were stained using the Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) (Catalog # BA1127) and counterstained with DAPI (blue).
all(9) 
IF analysis using anti- Calbindin antibody (A03047). detected in paraffin-embedded section of rat cerebellum tissue. The tissue section were stained using the Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) (Catalog # BA1127) and counterstained with DAPI (blue).
all(9) 
Flow Cytometry analysis of A431 cells using anti-Calbindin/CALB1 antibody (A03047).
Overlay histogram showing A431 cells stained with A03047 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Calbindin/CALB1 Antibody (A03047) at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample (Red line) was also used as a control.
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunofluorescence (IF) : | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Western blot analysis of Calbindin/CALB1 using anti-Calbindin/CALB1 antibody (A03047). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat kidney tissue lysates,
Lane 3: mouse brain tissue lysates,
Lane 4: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Calbindin/CALB1 antigen affinity purified polyclonal antibody (A03047) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Calbindin/CALB1 at approximately 28 kDa. The expected band size for Calbindin/CALB1 is at 30 kDa.
IHC analysis of Calbindin/CALB1 using anti-Calbindin/CALB1 antibody (A03047).
Calbindin/CALB1 was detected in a paraffin-embedded section of rat kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Calbindin/CALB1 Antibody (A03047) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Calbindin/CALB1 using anti-Calbindin/CALB1 antibody (A03047).
Calbindin/CALB1 was detected in a paraffin-embedded section of mouse brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Calbindin/CALB1 Antibody (A03047) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Calbindin/CALB1 using anti-Calbindin/CALB1 antibody (A03047).
Calbindin/CALB1 was detected in a paraffin-embedded section of mouse kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Calbindin/CALB1 Antibody (A03047) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Calbindin/CALB1 using anti-Calbindin/CALB1 antibody (A03047).
Calbindin/CALB1 was detected in a paraffin-embedded section of human cerebellum tissue. The tissue section was incubated with rabbit anti-Calbindin/CALB1 Antibody (A03047) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IF analysis using anti- Calbindin antibody (A03047). detected in paraffin-embedded section of rat cerebellum tissue. The tissue section were stained using the Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) (Catalog # BA1127) and counterstained with DAPI (blue).
IF analysis using anti- Calbindin antibody (A03047). detected in paraffin-embedded section of rat cerebellum tissue. The tissue section were stained using the Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) (Catalog # BA1127) and counterstained with DAPI (blue).
Flow Cytometry analysis of A431 cells using anti-Calbindin/CALB1 antibody (A03047).
Overlay histogram showing A431 cells stained with A03047 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Calbindin/CALB1 Antibody (A03047) at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Western blot analysis of Calbindin/CALB1 using anti-Calbindin/CALB1 antibody (A03047). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat kidney tissue lysates,
Lane 3: mouse brain tissue lysates,
Lane 4: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Calbindin/CALB1 antigen affinity purified polyclonal antibody (A03047) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Calbindin/CALB1 at approximately 28 kDa. The expected band size for Calbindin/CALB1 is at 30 kDa.
IHC analysis of Calbindin/CALB1 using anti-Calbindin/CALB1 antibody (A03047).
Calbindin/CALB1 was detected in a paraffin-embedded section of rat kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Calbindin/CALB1 Antibody (A03047) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Calbindin/CALB1 using anti-Calbindin/CALB1 antibody (A03047).
Calbindin/CALB1 was detected in a paraffin-embedded section of mouse brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Calbindin/CALB1 Antibody (A03047) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Calbindin/CALB1 using anti-Calbindin/CALB1 antibody (A03047).
Calbindin/CALB1 was detected in a paraffin-embedded section of mouse kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Calbindin/CALB1 Antibody (A03047) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Calbindin/CALB1 using anti-Calbindin/CALB1 antibody (A03047).
Calbindin/CALB1 was detected in a paraffin-embedded section of human cerebellum tissue. The tissue section was incubated with rabbit anti-Calbindin/CALB1 Antibody (A03047) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IF analysis using anti- Calbindin antibody (A03047). detected in paraffin-embedded section of rat cerebellum tissue. The tissue section were stained using the Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) (Catalog # BA1127) and counterstained with DAPI (blue).
IF analysis using anti- Calbindin antibody (A03047). detected in paraffin-embedded section of rat cerebellum tissue. The tissue section were stained using the Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) (Catalog # BA1127) and counterstained with DAPI (blue).
Flow Cytometry analysis of A431 cells using anti-Calbindin/CALB1 antibody (A03047).
Overlay histogram showing A431 cells stained with A03047 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Calbindin/CALB1 Antibody (A03047) at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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