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Anti-Calbindin/CALB1 Antibody

Rabbit polyclonal antibody

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筛选器: All WB IHC IP

PB9045

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Western blot analysis of Calbindin/CALB1 using anti-Calbindin/CALB1 antibody (PB9045). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human K562 whole cell lysates,
    Lane 2: rat kidney tissue lysates,
    Lane 3: rat brain tissue lysates,
    Lane 4: mouse kidney tissue lysates,
    Lane 5: mouse brain tissue lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Calbindin/CALB1 antigen affinity purified polyclonal antibody (PB9045) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Calbindin/CALB1 at approximately 26 kDa. The expected band size for Calbindin/CALB1 is at 30 kDa.

    all(7)
  • IHC analysis of Calbindin/CALB1 using anti-Calbindin/CALB1 antibody (PB9045) .
    Calbindin/CALB1 was detected in a paraffin-embedded section of mouse kidney tissue. The tissue section was incubated with rabbit anti-Calbindin/CALB1 Antibody (PB9045) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(7)
  • IHC analysis of Calbindin/CALB1 using anti-Calbindin/CALB1 antibody (PB9045) .
    Calbindin/CALB1 was detected in a paraffin-embedded section of rat kidney tissue. The tissue section was incubated with rabbit anti-Calbindin/CALB1 Antibody (PB9045) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(7)
  • IHC analysis of Calbindin/CALB1 using anti-Calbindin/CALB1 antibody (PB9045).
    Calbindin/CALB1 was detected in a paraffin-embedded section of human kidney tissue. The tissue section was incubated with rabbit anti-Calbindin/CALB1 Antibody (PB9045) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(7)
  • IHC analysis of Calbindin/CALB1 using anti-Calbindin/CALB1 antibody (PB9045).
    Calbindin/CALB1 was detected in a paraffin-embedded section of human cerebellum tissue. The tissue section was incubated with rabbit anti-Calbindin/CALB1 Antibody (PB9045) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(7)
  • IHC analysis of Calbindin/CALB1 using anti-Calbindin/CALB1 antibody (PB9045).
    Calbindin/CALB1 was detected in a paraffin-embedded section of human cerebellum tissue. The tissue section was incubated with rabbit anti-Calbindin/CALB1 Antibody (PB9045) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(7)
  • IP analysis of Calbindin/CALB1 using anti-Calbindin/CALB1 antibody (PB9045) in K562 whole cell lysate.
    Western blot analysis of Calbindin/CALB1 using anti- Calbindin/CALB1 antibody (PB9045).
    Lane 1: K562 whole cell lysates(30ug),
    Lane 2: Rabbit control IgG instead of anti- Calbindin/CALB1 antibody in K562 whole cell lysate,
    Lane 3: anti- Calbindin/CALB1 antibody (2μg) + K562 whole cell lysate (500μg).
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- Calbindin/CALB1 antigen affinity purified polyclonal antibody (PB9045) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Calbindin/CALB1 at approximately 26 kDa. The expected band size for Calbindin/CALB1 is at 30 kDa.

    all(7)

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产品简介>

产品名称
Anti-Calbindin/CALB1 Antibody
规格/价格
50μl|1280 100μl|2180 150μl|2800
指标别称
CAB27; CALB; CALB1; Calbindin; calbindin 1; 28kDa; Calbindin D28; Calbindin-D28k; D 28K
产品类型
Polyclonal
检验物种
human, mouse, rat
应用范围
WB, IHC, IP
基因名称
CALB1
宿主
Rabbit
抗体亚型
IgG
免疫原
E.coli-derived human Calbindin recombinant protein (Position: A2-E175). Human Calbindin shares 99% amino acid (aa) sequence identity with mouse and rat Calbindin.
计算分子量
30 kDa
实际分子量
26 kDa
成分
500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied.
背景资料
Calbindin is a calcium-binding protein belonging to the troponin C superfamily. Calretinin is expressed in central and peripheral nervous system and in many normal and pathological tissues. The rat and human calretinin exhibit 98% sequence homology and 91% homology to many other species. Two calcium binding proteins, calbindin and calretinin, have been reported to be expressed in abundance in Purkinje cells and other cell types in the cerebellum.
Uniprot ID
P05937  
RRID
文献引用格式
Calbindin/CALB1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB9045)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。
基因名全称
calbindin 1, 28kDa
蛋白名全称
Calbindin
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-400
ImmunoPrecipitation (IP):1:250-300
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

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    Anti-Calbindin/CALB1 Antibody

    筛选器: All WB IHC IP

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