Anti-Calbindin/CALB1 Antibody (Clone#CB-955)

筛选器： All WB IF IHC

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  Western blot analysis of Calbindin-D using anti-Calbindin-D antibody (BM0203).
  Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
  Lane 1: rat brain tissue lysates
  Lane 2: rat kidney tissue lysates
  Lane 3: mouse brain tissue lysates
  After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Calbindin-D antigen affinity purified monoclonal antibody (Catalog # BM0203) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Calbindin-D at approximately 28KD. The expected band size for Calbindin-D is at 28KD.

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  IF analysis of Calbindin-D using anti- Calbindin-D antibody (BM0203)
  Calbindin-D was detected in paraffin-embedded section of mouse brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL mouse anti- Calbindin-D Antibody (BM0203) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

• 

  Western blot analysis of Calbindin-D using anti-Calbindin-D antibody (BM0203).
  Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
  Lane 1: rat brain tissue lysates
  Lane 2: rat kidney tissue lysates
  Lane 3: mouse brain tissue lysates
  After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Calbindin-D antigen affinity purified monoclonal antibody (Catalog # BM0203) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Calbindin-D at approximately 28KD. The expected band size for Calbindin-D is at 28KD.

• 

  IF analysis of Calbindin-D using anti- Calbindin-D antibody (BM0203)
  Calbindin-D was detected in paraffin-embedded section of mouse brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL mouse anti- Calbindin-D Antibody (BM0203) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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