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Anti-FGFR1 Antibody

Rabbit polyclonal antibody

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筛选器: All WB IHC

BA0485

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Western blot analysis of FGFR1 using anti-FGFR1 antibody (BA0485). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: Rat Liver tissue lysates,
    Lane 2: Rat Brain tissue lysates,
    Lane 3: SMMC whole cell lysates,
    Lane 4: HT1080 whole cell lysates,
    Lane 5: MM231 whole cell lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-FGFR1 antigen affinity purified polyclonal antibody (BA0485) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for FGFR1 at approximately 92,140 kDa. The expected band size for FGFR1 is at 92 kDa.

    all(2)
  • IHC analysis of FGFR1 using anti-FGFR1 antibody (BA0485).
    FGFR1 was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was incubated with rabbit anti-FGFR1 Antibody (BA0485) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(2)

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产品名称
Anti-FGFR1 Antibody
规格/价格
50μl|1280 100μl|2180 150μl|2800
指标别称
bFGF R 1; BFGFR; CD331; CEK; FGFBR; FGFR 1; FGFR1; FLG; FLT 2; FLT2; Fms like tyrosine kinase 2; HBGFR; KAL2; N SAM; OGD; Proto oncogene c Fgr
产品类型
Polyclonal
检验物种
human, mouse, rat
应用范围
WB, IHC
基因名称
FGFR1
宿主
Rabbit
抗体亚型
IgG
免疫原
A synthetic peptide corresponding to a sequence at the C-terminus of human FGFR1, different from the related mouse sequence by two amino acids and rat sequence by three amino acids.
计算分子量
92 kDa
实际分子量
92,140 kDa
成分
500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied.
背景资料
FGFR1, Fibroblast growth factor receptor 1, also known as basic fibroblast growth factor receptor 1, fms-related tyrosine kinase-2/Pfeiffer syndrome, and CD331, is a receptor tyrosine kinase whose ligands are specific members of the fibroblast growth factor family. The FGFR1 gene is localized to 8p12-p11.2 by in situ hybridization. FGFR1 is essential for the normal formation of the organ of Corti and that phenotype severity observed in FGFR1 mutants is dependent on the dose of FGFR1.Mutations in this gene have been associated with Pfeiffer syndrome, Jackson-Weiss syndrome, Antley-Bixler syndrome, osteoglophonic dysplasia, squamous cell lung cancer and autosomal dominant Kallmann syndrome 2.
Uniprot ID
P11362  
文献引用格式
FGFR1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA0485)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。
基因名全称
fibroblast growth factor receptor 1
蛋白名全称
Fibroblast growth factor receptor 1(FGFR-1/bFGF-R-1)
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-400
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

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    Anti-FGFR1 Antibody

    筛选器: All WB IHC

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