Anti-SMAD1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC ICC/IF

BA3067

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of SMAD1 using anti-SMAD1 antibody (BA3067). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: SMMC whole cell lysates,
Lane 2: K562 whole cell lysates,
Lane 3: HT1080 whole cell lysates,
Lane 4: HELA whole cell lysates,
Lane 5: JURKAT whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SMAD1 antigen affinity purified polyclonal antibody (BA3067) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for SMAD1 at approximately 52 kDa. The expected band size for SMAD1 is at 52 kDa.
all(3)
IHC analysis of SMAD1 using anti-SMAD1 antibody (BA3067).
SMAD1 was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was incubated with rabbit anti-SMAD1 Antibody (BA3067) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(3)
ICC analysis of SMAD1 using anti- SMAD1 antibody (BA3067).
SMAD1 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-SMAD1 Antibody (BA3067) at a dilution of 1:100. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(3)

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产品简介>

产品名称

Anti-SMAD1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

BSP 1; BSP1; hSMAD1; JV4 1; JV41; MAD homolog 1; Mad related protein 1; MADH1; MADR1; Mothers against DPP homolog 1; SMAD 1; SMAD family member 1; SMAD1

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF

基因名称

SMAD1

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of mouse Smad1, different from the related rat sequence by one amino acid, and different from the related human sequence by two amino acids.

计算分子量

52 kDa

实际分子量

52 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Mothers against decapentaplegic homolog 1 also known as SMAD family member 1 or SMAD1 is a protein that in humans is encoded by the SMAD1 gene. SMAD1 belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene "mothers against decapentaplegic"(Mad) and the C. elegans gene Sma. The gene was assigned to human chromosome 4q31.21. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways.

Uniprot ID

P70340

文献引用格式

SMAD1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA3067)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

SMAD family member 1

蛋白名全称

Mothers against decapentaplegic homolog 1

实验方案>

实验步骤

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