Anti-SMAD1 Antibody (Clone#CAC-19)

Rabbit monoclonal antibody

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筛选器： All WB IHC ICC/IF

BM4171

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-SMAD1 antibody (BM4171). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human SIHA whole cell lysates,
Lane 4: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SMAD1 antigen affinity purified monoclonal antibody (BM4171) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for SMAD1 at approximately 52 kDa. The expected band size for SMAD1 is at 52 kDa.
all(3)
Immunohistochemical analysis of paraffin-embedded human kidney, using Smad1 Antibody.
all(3)
Immunofluorescent analysis using the Antibody.
all(3)

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产品名称

Anti-SMAD1 Antibody (Clone#CAC-19)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

BSP 1; BSP1; hSMAD1; JV4 1; JV41; MAD homolog 1; Mad related protein 1; MADH1; MADR1; Mothers against DPP homolog 1; SMAD 1; SMAD family member 1; SMAD1

产品类型

Monoclonal

检验物种

human, mouse

应用范围

WB, IHC, ICC/IF

基因名称

SMAD1

克隆号

CAC-19

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human Smad1

计算分子量

52 kDa

实际分子量

52 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

SMADs are proteins that modulate the activity of transforming growth factor beta ligands. The SMADs, often in complex with other SMADs/CoSMAD, act as transcription factors that regulate the expression of certain genes. It was concluded that targeted ubiquitination of SMADs may serve to control both embryonic development and a wide variety of cellular responses to TGF-beta signals. R-Smads or receptor regulated Smads are a class of proteins that include SMAD1, SMAD2, SMAD3, SMAD5, and SMAD8. In response to signals by the TGF-β superfamily of ligands these proteins associate with receptor kinases and are phosphorylated at an SSXS motif at their extreme C-terminus. These proteins then typically bind to the common mediator Smad or co-SMAD SMAD4.

Uniprot ID

Q15797

文献引用格式

SMAD1 Antibody (Clone#CAC-19) (Boster Biological Technology, Wuhan, China. Catalog#BM4171)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Mothers against decapentaplegic homolog 1

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实验步骤

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