Anti-SMAD1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB

PB0444

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of SMAD1 using anti-SMAD1 antibody (PB0444). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat Cardiac Muscle tissue lysates,
Lane 2: Mouse Cardiac Muscle tissue lysates,
Lane 3: Rat Skeletal Muscle tissue lysates,
Lane 4: Mouse Skeletal Muscle tissue lysates,
Lane 5: 293T whole cell lysates,
Lane 6: MCF-7 whole cell lysates,
Lane 7: HELA whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SMAD1 antigen affinity purified polyclonal antibody (PB0444) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for SMAD1 at approximately 52 kDa. The expected band size for SMAD1 is at 52 kDa.
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产品名称

Anti-SMAD1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

BSP 1; BSP1; hSMAD1; JV4 1; JV41; MAD homolog 1; Mad related protein 1; MADH1; MADR1; Mothers against DPP homolog 1; SMAD 1; SMAD family member 1; SMAD1

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

基因名称

SMAD1

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence in the middle region of human SMAD1 different from the related mouse sequence by two amino acids, and from the related rat sequence by five amino acids.

计算分子量

52 kDa

实际分子量

52 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

SMADs are proteins that modulate the activity of transforming growth factor beta ligands. The SMADs, often in complex with other SMADs/CoSMAD, act as transcription factors that regulate the expression of certain genes. It was concluded that targeted ubiquitination of SMADs may serve to control both embryonic development and a wide variety of cellular responses to TGF-beta signals. R-Smads or receptor regulated Smads are a class of proteins that include SMAD1, SMAD2, SMAD3, SMAD5, and SMAD8. In response to signals by the TGF-β superfamily of ligands these proteins associate with receptor kinases and are phosphorylated at an SSXS motif at their extreme C-terminus. These proteins then typically bind to the common mediator Smad or co-SMAD SMAD4.

Uniprot ID

Q15797

文献引用格式

SMAD1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB0444)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

SMAD family member 1

蛋白名全称

Mothers against decapentaplegic homolog 1

实验方案>

实验步骤

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