Anti-SMAD1 Antibody (Clone#OTI2D2)

Mouse monoclonal antibody

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筛选器： All IHC WB

M00728-4

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-SMAD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, M00728-4)
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SMAD1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SMAD1 (1:500).
all(2)

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产品简介>

产品名称

Anti-SMAD1 Antibody (Clone#OTI2D2)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

BSP 1; BSP1; hSMAD1; JV4 1; JV41; MAD homolog 1; Mad related protein 1; MADH1; MADR1; Mothers against DPP homolog 1; SMAD 1; SMAD family member 1; SMAD1

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

IHC, WB

基因名称

SMAD1

克隆号

OTI2D2

宿主

Mouse

抗体亚型

IgG1

免疫原

Full length human recombinant protein of human SMAD1(NP_001003688) produced in HEK293T cell.

计算分子量

52 kDa

实际分子量

52 kDa

成分

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]

Uniprot ID

Q15797

文献引用格式

SMAD1 Antibody (Clone#OTI2D2) (Boster Biological Technology, Wuhan, China. Catalog#M00728-4)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

SMAD family member 1/2/3/5

蛋白名全称

Mothers against decapentaplegic homolog 1/2/3/5

实验方案>

实验步骤

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