Anti-XRCC1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC ICC/IF

BA3670

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of XRCC1 using anti-XRCC1 antibody (BA3670). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Hela whole cell lysates,
Lane 2: 293T whole cell lysates,
Lane 3: K562 whole cell lysates,
Lane 4: Jurkat whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-XRCC1 antigen affinity purified polyclonal antibody (BA3670) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for XRCC1 at approximately 90 kDa. The expected band size for XRCC1 is at 69 kDa.
all(3)
IHC analysis of XRCC1 using anti-XRCC1 antibody (BA3670).
XRCC1 was detected in a paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-XRCC1 Antibody (BA3670) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(3)
ICC/IF analysis of XRCC1 using anti-XRCC1 antibody (BA3670) and anti-Beta Tubulin antibody (M01857-3).
XRCC1 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-XRCC1 Antibody (BA3670) at a dilution of 1:100. Cy3-Conjugated Anti-rabbit IgG Secondary Antibody (Red) (Catalog # BA1032) and Fluoro488-conjugated Anti-mouse IgG Secondary Antibody (Green) (Catalog # BA1126) were used as secondary antibody.
all(3)

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产品简介>

产品名称

Anti-XRCC1 Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

DNA repair protein XRCC1; RCC; XRCC1

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF

基因名称

XRCC1

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the N-terminus of human XRCC1, identical to the related mouse sequence, and different from the related rat sequence by one amino acid.

计算分子量

69 kDa

实际分子量

90 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

XRCC1(X-RAY REPAIR, COMPLEMENTING DEFECTIVE, IN CHINESE HAMSTER, 1) is a DNA repair protein which complexes with DNA ligase III. The protein encoded by this gene is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. The XRCC1 gene is mapped to 19q13.31. The XRCC1 interacts with DNA ligase III, polymerase beta and poly(ADP-ribose) polymerase to participate in the base excision repair pathway. It may play a role in DNA processing during meiogenesis and recombination in germ cells. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. XRCC1 is phosphorylated in vivo and in vitro by CK2, and CK2 phosphorylation of XRCC1 on ser518, thr519, and thr523 largely determines aprataxin binding to XRCC1 through its FHA domain.

Uniprot ID

P18887

文献引用格式

XRCC1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA3670)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

X-ray repair complementing defective repair in Chinese hamster cells 1

蛋白名全称

DNA repair protein XRCC1

实验方案>

实验步骤

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