Anti-Cytokeratin 18/KRT18 Antibody (Clone#CY-90)

Mouse monoclonal antibody

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筛选器： All WB IHC IF

BM0032

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti- KRT18 antibody (BM0032). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: A431 whole cell lysates,
Lane 3: Caco-2 whole cell lysates,
Lane 4: HepG2 whole cell lysates.
Use mouse anti- KRT18 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for KRT18 at approximately 48KD. The expected band size for KRT18 is at 48KD.
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产品简介>

产品名称

Anti-Cytokeratin 18/KRT18 Antibody (Clone#CY-90)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

CK 18; CK18; CYK18; Cytokeratin 18; K18; keratin 18; KRT18

产品类型

Monoclonal

检验物种

human, rat

应用范围

WB, IHC, IF

基因名称

KRT18

克隆号

CY-90

宿主

Mouse

抗体亚型

IgG1

免疫原

The human epidermal carcinoma A-431 and MCF-7 human breast cancer cell lines.

计算分子量

48 kDa

实际分子量

48 kDa

成分

200ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA and 50% glycerol.

纯化方式

Ascites

浓度

200 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Intermediate filaments(IFs) are a structurally related family of cellular proteins that appear to be intimately involved with the cytoskeleton. Human keratin 18(KRT18) and the homologous mouse Endo B are type I IF protein subunits whose expression is restricted in adults to a variety of simple epithelial tissues. The KRT18 gene is 3,791 bp long and the keratin 18 protein is coded for by 7 exons. The K18 gene is 3791 bp in length and the K18 protein is coded for by seven exons. By Southern blotting using the genomic DNA PCR product, the gene for keratin 18 is assigned to chromosome 12. Mutation of human keratin 18 in association with cryptogenic cirrhosis.

Uniprot ID

P05783

文献引用格式

Cytokeratin 18/KRT18 Antibody (Clone#CY-90) (Boster Biological Technology, Wuhan, China. Catalog#BM0032)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

keratin 18

蛋白名全称

Keratin, type I cytoskeletal 18

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实验步骤

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