Anti-Cytokeratin 18/KRT18 Antibody (Clone#GBF-11)

Rabbit monoclonal antibody

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筛选器： All WB IHC ICC/IF FCM

BM4594

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-Cytokeratin 18/KRT18 antibody (BM4594). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human HT-1080 whole cell lysates,
Lane 4: human U2OS whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: rat thymus tissue lysates,
Lane 7: mouse liver tissue lysates,
Lane 8: mouse thymus tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Cytokeratin 18/KRT18 antigen affinity purified monoclonal antibody (BM4594) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Cytokeratin 18/KRT18 at approximately 48 kDa. The expected band size for Cytokeratin 18/KRT18 is at 48 kDa.
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IHC analysis of Cytokeratin 18/KRT18 using anti-Cytokeratin 18/KRT18 antibody (BM4594) .
Cytokeratin 18/KRT18 was detected in a paraffin-embedded section of human placenta tissue. The tissue section was incubated with rabbit anti-Cytokeratin 18/KRT18 Antibody (BM4594) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(11)
IHC analysis of Cytokeratin 18/KRT18 using anti-Cytokeratin 18/KRT18 antibody (BM4594) .
Cytokeratin 18/KRT18 was detected in a paraffin-embedded section of mouse bladder tissue. The tissue section was incubated with rabbit anti-Cytokeratin 18/KRT18 Antibody (BM4594) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(11)
IHC analysis of Cytokeratin 18/KRT18 using anti-Cytokeratin 18/KRT18 antibody (BM4594) .
Cytokeratin 18/KRT18 was detected in a paraffin-embedded section of mouse bladder tissue. The tissue section was incubated with rabbit anti-Cytokeratin 18/KRT18 Antibody (BM4594) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(11)
IHC analysis of Cytokeratin 18/KRT18 using anti-Cytokeratin 18/KRT18 antibody (BM4594) .
Cytokeratin 18/KRT18 was detected in a paraffin-embedded section of rat bladder tissue. The tissue section was incubated with rabbit anti-Cytokeratin 18/KRT18 Antibody (BM4594) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(11)
IHC analysis of Cytokeratin 18/KRT18 using anti-Cytokeratin 18/KRT18 antibody (BM4594) .
Cytokeratin 18/KRT18 was detected in a paraffin-embedded section of rat bladder tissue. The tissue section was incubated with rabbit anti-Cytokeratin 18/KRT18 Antibody (BM4594) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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Immunofluorescent analysis using the Antibody.
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Immunofluorescent analysis using the Antibody.
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Immunofluorescent analysis using the Antibody.
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Immunofluorescent analysis of pc-12 cells, using Cytokeratin 18 Antibody.
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产品简介>

产品名称

Anti-Cytokeratin 18/KRT18 Antibody (Clone#GBF-11)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

CK 18; CK18; CYK18; Cytokeratin 18; K18; keratin 18; KRT18

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, FCM

基因名称

KRT18

克隆号

GBF-11

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human Cytokeratin 18.

计算分子量

48 kDa

实际分子量

48 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Intermediate filaments(IFs) are a structurally related family of cellular proteins that appear to be intimately involved with the cytoskeleton. Human keratin 18(KRT18) and the homologous mouse Endo B are type I IF protein subunits whose expression is restricted in adults to a variety of simple epithelial tissues. The KRT18 gene is 3,791 bp long and the keratin 18 protein is coded for by 7 exons. The K18 gene is 3791 bp in length and the K18 protein is coded for by seven exons. By Southern blotting using the genomic DNA PCR product, the gene for keratin 18 is assigned to chromosome 12. Mutation of human keratin 18 in association with cryptogenic cirrhosis.

Uniprot ID

P05783

文献引用格式

Cytokeratin 18/KRT18 Antibody (Clone#GBF-11) (Boster Biological Technology, Wuhan, China. Catalog#BM4594)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Keratin, type I cytoskeletal 18

实验方案>

实验步骤

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