产品名称
Anti-Cytokeratin 7/KRT7 Antibody (Clone#LDS-68)
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
Cell and organelle markers; CK 7; CK7; Cytokeratin 7; Cytokeratin 7-specific; Cytoskeleton Marker; K2C7; K7; keratin 7; KRT7; Sarcolectin; SCL; Type II keratin Kb7
免疫原
Cytoskeletal preparation of the RT4 human bladder carcinoma cell line.
成分
200ug/ml antibody with PBS ,0.02% NaN3 , 1mg BSA and 50% glycerol.
保存条件
12 months from date of receipt,-20℃ as supplied.
背景资料
KRT7 is a type II keratin of simple nonkeratinizing epithelia. The deduced 489-amino acid protein has a calculated molecular mass of about 54 kD. K7 contains 4 central alpha-helical segments with heptad repeats of hydrophobic residues characteristic of a coiled-coil region. Within this domain, K7 shares 73% homology with epidermal K6B. KRT7 gene contains 9 exons and spans more than 15.6 kb. K7 gene is mapped to chromosome 12. Keratin 7 is expressed in a wide range of epithelial structures in humans.
文献引用格式
Cytokeratin 7/KRT7 Antibody (Clone#LDS-68) (Boster Biological Technology, Wuhan, China. Catalog#BM1618)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.
蛋白名全称
Keratin, type II cytoskeletal 7
推荐稀释比
Western blot (WB): | 1:500-2000 |
Immunohistochemistry (IHC): | 1:50-400 |
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |