Anti-Cytokeratin 7/KRT7 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC

A02416-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of Cytokeratin 7/KRT7 using anti-Cytokeratin 7/KRT7 antibody (A02416-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Hela whole cell lysates,
Lane 2: RT4 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Cytokeratin 7/KRT7 antigen affinity purified polyclonal antibody (A02416-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Cytokeratin 7/KRT7 at approximately 51 kDa. The expected band size for Cytokeratin 7/KRT7 is at 51 kDa.
all(4)
IHC analysis of Cytokeratin 7/KRT7 using anti-Cytokeratin 7/KRT7 antibody (A02416-2).
Cytokeratin 7/KRT7 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-Cytokeratin 7/KRT7 Antibody (A02416-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(4)
IHC analysis of Cytokeratin 7/KRT7 using anti-Cytokeratin 7/KRT7 antibody (A02416-2).
Cytokeratin 7/KRT7 was detected in a paraffin-embedded section of human placenta tissue. The tissue section was incubated with rabbit anti-Cytokeratin 7/KRT7 Antibody (A02416-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(4)
IHC analysis of Cytokeratin 7/KRT7 using anti-Cytokeratin 7/KRT7 antibody (A02416-2).
Cytokeratin 7/KRT7 was detected in a paraffin-embedded section of human thyroid cancer tissue. The tissue section was incubated with rabbit anti-Cytokeratin 7/KRT7 Antibody (A02416-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(4)

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产品简介>

产品名称

Anti-Cytokeratin 7/KRT7 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Cell and organelle markers; CK 7; CK7; Cytokeratin 7; Cytokeratin 7-specific; Cytoskeleton Marker; K2C7; K7; keratin 7; KRT7; Sarcolectin; SCL; Type II keratin Kb7

产品类型

Polyclonal

检验物种

human

应用范围

WB, IHC

基因名称

KRT7

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence in the middle region of human KRT7, which shares 91.3% amino acid (aa) sequence identity with mouse and rat KRT7.

计算分子量

51 kDa

实际分子量

51 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Keratin, type II cytoskeletal 7 also known as cytokeratin-7 (CK-7) or keratin-7 (K7) or sarcolectin (SCL) is a protein that in humans is encoded by the KRT7 gene. The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described.

Uniprot ID

P08729

文献引用格式

Cytokeratin 7/KRT7 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A02416-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

keratin 7

蛋白名全称

Keratin, type II cytoskeletal 7

实验方案>

实验步骤

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