Anti-E-cadherin/CDH1 Antibody (Clone#EA-20)

Rabbit monoclonal antibody

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筛选器： All WB IHC ICC/IF FCM

BM3903

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-E-cadherin/CDH1 antibody (BM3903). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human T-47D whole cell lysates,
Lane 3: human Hacat whole cell lysates,
Lane 4: human RT4 whole cell lysates,
Lane 5: human Caco-2 whole cell lysates,
Lane 6: human A431 whole cell lysates,
Lane 7: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-E-cadherin/CDH1 antigen affinity purified monoclonal antibody (BM3903) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for E-cadherin/CDH1 at approximately 130 kDa. The expected band size for E-cadherin/CDH1 is at 90 kDa.
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IHC analysis of E-cadherin/CDH1 using anti-E-cadherin/CDH1 antibody (BM3903) .
E-cadherin/CDH1 was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-E-cadherin/CDH1 Antibody (BM3903) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of E-cadherin/CDH1 using anti-E-cadherin/CDH1 antibody (BM3903) .
E-cadherin/CDH1 was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was incubated with rabbit anti-E-cadherin/CDH1 Antibody (BM3903) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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Immunofluorescent analysis using the Antibody.
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产品简介>

产品名称

Anti-E-cadherin/CDH1 Antibody (Clone#EA-20)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Arc 1; Cadherin 1; CD324; CDH1; CDHE; E cadherin; ECAD; E-cad; E-cadherin; LCAM; UVO; Uvomorulin

产品类型

Monoclonal

检验物种

human

应用范围

WB, IHC, ICC/IF, FCM

基因名称

CDH1

克隆号

EA-20

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human E Cadherin

计算分子量

97 kDa

实际分子量

130 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

CDH1 (Cadherin 1), also known as ECAD or UVO, is a protein that in humans is encoded by the CDH1 gene. Cadherin-1 is a classical member of the cadherin superfamily. By Southern analysis of DNA from a panel of mouse-human somatic cell hybrids, Mansouri et al. (1987, 1988) assigned the UVO gene to 16q (16p11-qter). Frebourg et al. (2006) found that in human embryos CDH1 is highly expressed at 4 and 5 weeks in the frontonasal prominence and at 6 weeks in the lateral and medial nasal prominences, and is therefore expressed during critical stages of lip and palate development. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.

Uniprot ID

P12830

文献引用格式

E-cadherin/CDH1 Antibody (Clone#EA-20) (Boster Biological Technology, Wuhan, China. Catalog#BM3903)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Cadherin-1

实验方案>

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