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Anti-E-cadherin/CDH1 Antibody

Rabbit polyclonal antibody

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筛选器: All WB IHC ICC/IF IF

PB9561

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Western blot analysis of anti- CDH1/E-cadherin antibody (PB9561). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
    Lane 1: human MCF-7 whole cell lysates,
    Lane 2: human A431 whole cell lysates.
    Use rabbit anti- CDH1/E-cadherin 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for CDH1/E-cadherin at approximately 130KD. The expected band size for CDH1/E-cadherin is at 97KD.

    all(11)
  • IHC analysis of E-cadherin/CDH1 using anti-E-cadherin/CDH1 antibody (PB9561).
    E-cadherin/CDH1 was detected in a paraffin-embedded section of human placenta tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-E-cadherin/CDH1 Antibody (PB9561) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

    all(11)
  • IHC analysis of E-cadherin/CDH1 using anti-E-cadherin/CDH1 antibody (PB9561).
    E-cadherin/CDH1 was detected in frozen section of human placenta tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-E-cadherin/CDH1 Antibody (PB9561) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

    all(11)
  • IHC analysis of E-cadherin/CDH1 using anti-E-cadherin/CDH1 antibody (PB9561).
    E-cadherin/CDH1 was detected in a paraffin-embedded section of mouse intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-E-cadherin/CDH1 Antibody (PB9561) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

    all(11)
  • IHC analysis of E-cadherin/CDH1 using anti-E-cadherin/CDH1 antibody (PB9561).
    E-cadherin/CDH1 was detected in a paraffin-embedded section of rat intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-E-cadherin/CDH1 Antibody (PB9561) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

    all(11)
  • IHC analysis of E-cadherin/CDH1 using anti-E-cadherin/CDH1 antibody (PB9561).
    E-cadherin/CDH1 was detected in frozen section of mouse intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-E-cadherin/CDH1 Antibody (PB9561) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

    all(11)
  • IHC analysis of E-cadherin/CDH1 using anti-E-cadherin/CDH1 antibody (PB9561).
    E-cadherin/CDH1 was detected in frozen section of rat intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-E-cadherin/CDH1 Antibody (PB9561) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

    all(11)
  • IHC analysis of E-cadherin/CDH1 using anti-E-cadherin/CDH1 antibody (PB9561).
    E-cadherin/CDH1 was detected in frozen section of human placenta tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-E-cadherin/CDH1 Antibody (PB9561) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

    all(11)
  • IF analysis of E-cadherin/CDH1 using anti-E-cadherin/CDH1 antibody (PB9561).
    E-cadherin/CDH1 was detected in an immunocytochemical section of MCF-7 cells. The section was incubated with rabbit anti-E-cadherin/CDH1 Antibody (PB9561) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

    all(11)
  • IF analysis using anti- E Cadherin antibody (PB9561). detected in paraffin-embedded section of mouse colon tissue. The tissue section were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green) (Catalog # BA1127) and counterstained with DAPI (blue).

    all(11)
  • IF analysis using anti- E Cadherin antibody (PB9561). detected in paraffin-embedded section of human colon cancer tissue. The tissue section were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green) (Catalog # BA1127) and counterstained with DAPI (blue).

    all(11)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称
Anti-E-cadherin/CDH1 Antibody
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
Arc 1; Cadherin 1; CD324; CDH1; CDHE; E cadherin; ECAD; E-cad; E-cadherin; LCAM; UVO; Uvomorulin
产品类型
Polyclonal
检验物种
human, mouse, rat
应用范围
WB, IHC, ICC/IF, IF
基因名称
CDH1
宿主
Rabbit
抗体亚型
IgG
免疫原
E.coli-derived human E Cadherin recombinant protein (Position: A286-A703). Human E Cadherin shares 79.7% and 80.9% amino acid (aa) sequence identity with mouse and rat E Cadherin, respectively.
计算分子量
97 kDa
实际分子量
130 kDa
成分
500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
CDH1 (Cadherin 1), also known as ECAD or UVO, is a protein that in humans is encoded by the CDH1 gene. Cadherin-1 is a classical member of the cadherin superfamily. By Southern analysis of DNA from a panel of mouse-human somatic cell hybrids, Mansouri et al. (1987, 1988) assigned the UVO gene to 16q (16p11-qter). Frebourg et al. (2006) found that in human embryos CDH1 is highly expressed at 4 and 5 weeks in the frontonasal prominence and at 6 weeks in the lateral and medial nasal prominences, and is therefore expressed during critical stages of lip and palate development. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.
Uniprot ID
P12830  
RRID
文献引用格式
E-cadherin/CDH1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB9561)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。
基因名全称
cadherin 1, type 1, E-cadherin (epithelial)
蛋白名全称
Cadherin-1
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-400
Immunocytochemistry/Immunofluorescence (ICC/IF):1:50-400
Immunofluorescence (IF):1:50-400
ELISA:1:100-1000
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

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    Anti-E-cadherin/CDH1 Antibody

    筛选器: All WB IHC ICC/IF IF

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