Anti-HDAC2 Antibody (Clone#AAE-8)

Rabbit monoclonal antibody

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筛选器： All WB IHC ICC/IF IP FCM

BM3967

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of HDAC2 using anti-HDAC2 antibody (BM3967). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HL-60 whole cell lysates,
Lane 2: human THP-1 whole cell lysates,
Lane 3: human U937 whole cell lysates,
Lane 4: human Caco-2 whole cell lysates,
Lane 5: human U2OS whole cell lysates,
Lane 6: human A431 whole cell lysates,
Lane 7: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HDAC2 antigen affinity purified monoclonal antibody (BM3967) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for HDAC2 at approximately 60 kDa. The expected band size for HDAC2 is at 55 kDa.
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IHC analysis of HDAC2 using anti-HDAC2 antibody (BM3967).
HDAC2 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-HDAC2 Antibody (BM3967) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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产品简介>

产品名称

Anti-HDAC2 Antibody (Clone#AAE-8)

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

HD2; HDAC2; histone deacetylase 2; RPD3; YAF1

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, IP, FCM

基因名称

HDAC2

克隆号

AAE-8

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human HDAC2

计算分子量

55 kDa

实际分子量

60 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Histone deacetylase 2 is an enzyme that in humans is encoded by the HDAC2 gene. This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes and are responsible for the deacetylation of lysine residues on the N-terminal region of the core histones(H2A, H2B, H3 and H4). This protein also forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Betz et al.(1998) performed PCR using HDAC2-specific primers to screen a somatic cell hybrid mapping panel. They mapped the HDAC2 gene to human chromosome 6q21, a region of the genome altered in some cancers, including retinoblastoma.

Uniprot ID

Q92769

文献引用格式

HDAC2 Antibody (Clone#AAE-8) (Boster Biological Technology, Wuhan, China. Catalog#BM3967)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Histone deacetylase 2

实验方案>

实验步骤

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