Western blot (WB): | 1:500-2000 |
Immunohistochemistry (IHC): | 1:50-200 |
Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-200 |
Flow Cytometry (FCM): | 1:50 |
Western blot analysis of anti-Vimentin/VIM antibody (BM4029). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human U-87MG whole cell lysates,
Lane 3: human U251 whole cell lysates,
Lane 4: human Jurkat whole cell lysates,
Lane 5: rat lung tissue lysates,
Lane 6: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Vimentin/VIM antigen affinity purified monoclonal antibody (BM4029) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Vimentin/VIM at approximately 54 kDa. The expected band size for Vimentin/VIM is at 54 kDa.
IHC analysis of Vimentin/VIM using anti-Vimentin/VIM antibody (BM4029) .
Vimentin/VIM was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-Vimentin/VIM Antibody (BM4029) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Vimentin/VIM using anti-Vimentin/VIM antibody (BM4029) .
Vimentin/VIM was detected in a paraffin-embedded section of human glioma tissue. The tissue section was incubated with rabbit anti-Vimentin/VIM Antibody (BM4029) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Vimentin/VIM using anti-Vimentin/VIM antibody (BM4029) .
Vimentin/VIM was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-Vimentin/VIM Antibody (BM4029) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Vimentin/VIM using anti-Vimentin/VIM antibody (BM4029) .
Vimentin/VIM was detected in a paraffin-embedded section of human non small cell lung cancer tissue. The tissue section was incubated with rabbit anti-Vimentin/VIM Antibody (BM4029) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Vimentin/VIM using anti-Vimentin/VIM antibody (BM4029) .
Vimentin/VIM was detected in a paraffin-embedded section of human ovarian cancer tissue. The tissue section was incubated with rabbit anti-Vimentin/VIM Antibody (BM4029) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Vimentin/VIM using anti-Vimentin/VIM antibody (BM4029) .
Vimentin/VIM was detected in a paraffin-embedded section of human placenta tissue. The tissue section was incubated with rabbit anti-Vimentin/VIM Antibody (BM4029) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Vimentin/VIM using anti-Vimentin/VIM antibody (BM4029) .
Vimentin/VIM was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was incubated with rabbit anti-Vimentin/VIM Antibody (BM4029) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
Immunofluorescent analysis of Hela cells, using Vimentin Antibody.
Western blot analysis of anti-Vimentin/VIM antibody (BM4029). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human U-87MG whole cell lysates,
Lane 3: human U251 whole cell lysates,
Lane 4: human Jurkat whole cell lysates,
Lane 5: rat lung tissue lysates,
Lane 6: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Vimentin/VIM antigen affinity purified monoclonal antibody (BM4029) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Vimentin/VIM at approximately 54 kDa. The expected band size for Vimentin/VIM is at 54 kDa.
IHC analysis of Vimentin/VIM using anti-Vimentin/VIM antibody (BM4029) .
Vimentin/VIM was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-Vimentin/VIM Antibody (BM4029) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Vimentin/VIM using anti-Vimentin/VIM antibody (BM4029) .
Vimentin/VIM was detected in a paraffin-embedded section of human glioma tissue. The tissue section was incubated with rabbit anti-Vimentin/VIM Antibody (BM4029) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Vimentin/VIM using anti-Vimentin/VIM antibody (BM4029) .
Vimentin/VIM was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-Vimentin/VIM Antibody (BM4029) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Vimentin/VIM using anti-Vimentin/VIM antibody (BM4029) .
Vimentin/VIM was detected in a paraffin-embedded section of human non small cell lung cancer tissue. The tissue section was incubated with rabbit anti-Vimentin/VIM Antibody (BM4029) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Vimentin/VIM using anti-Vimentin/VIM antibody (BM4029) .
Vimentin/VIM was detected in a paraffin-embedded section of human ovarian cancer tissue. The tissue section was incubated with rabbit anti-Vimentin/VIM Antibody (BM4029) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Vimentin/VIM using anti-Vimentin/VIM antibody (BM4029) .
Vimentin/VIM was detected in a paraffin-embedded section of human placenta tissue. The tissue section was incubated with rabbit anti-Vimentin/VIM Antibody (BM4029) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Vimentin/VIM using anti-Vimentin/VIM antibody (BM4029) .
Vimentin/VIM was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was incubated with rabbit anti-Vimentin/VIM Antibody (BM4029) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
Immunofluorescent analysis of Hela cells, using Vimentin Antibody.